根皮苷在AOPPs诱导的滋养细胞衰老中的保护作用  

Protective effect of phlorizin on AOPPs-induced trophoblast cells senescence

作　　者：李正娟 梅雪然 李莉平 LI Zhengjuan;MEI Xueran;LI Liping(Department of Obstetrics and Gynecology,The Second Affiliated Hospital,South China University of Technology,Guangdong Guangzhou 510006,China;Department of Obstetrics,Shenzhen People's Hospital/The Second Clinical Medical College,Jinan University/The First Affiliated Hospital,Southern University of Science and Technology,Guangdong Shenzhen 518020,China)

机构地区：[1]华南理工大学附属第二医院妇产科,广东广州510006 [2]深圳市人民医院/暨南大学第二临床医学院/南方科技大学第一附属医院产科,广东深圳518020

出　　处：《中国医院药学杂志》2023年第19期2142-2147,共6页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:81871217);国家自然科学基金青年基金资助项目(编号:82204054)。

摘　　要：目的:探讨根皮苷(phlorizin, PZ)在晚期氧化蛋白产物(advanced oxidative protein products, AOPPs)诱导的滋养细胞衰老中的保护作用及其相应的机制。方法:根皮苷(20μg·mL-1)和AOPPs(200μg·mL-1)共处理滋养细胞系HTR-8/SV neo细胞48 h。通过检测细胞周期、衰老相关β-半乳糖苷酶(senescence-associated β-galactosidase, SA β-Gal)、线粒体膜电位(ΔΨm)、衰老相关异染色质病灶(senescence-associated heterochromatin foci, SAHF)分析HTR-8/SV neo细胞衰老情况。使用流式细胞术检测细胞内活性氧(reactive oxygen species, ROS)水平,蛋白质印迹法(WB)检测蛋白p53、p21的表达水平。结果:和AOPPs组相比,根皮苷和AOPPs共处理后显著降低了处于G0/G1期细胞的百分比(P<0.01)、SA β-Gal和SAHF的表达水平(P均<0.01),显著增加了处于G2期细胞的百分比(P<0.05)和细胞ΔΨm(P<0.01)。流式检测结果表明,AOPPs处理组的细胞内ROS水平显著高于对照组(P<0.01),而根皮苷和AOPPs共处理组的细胞内ROS水平显著低于AOPPs处理组(P<0.01)。同时,根皮苷和AOPPs共处理后p53蛋白和p21蛋白的相对表达水平显著低于AOPPs组(均为P<0.01)。结论:根皮苷具有抗氧化能力,并且可以逆转AOPPs对滋养细胞的促衰老作用。OBJECTIVE To investigate the protective effect of phlorizin(PZ)on trophoblast cells senescence induced by advanced oxidative protein products(AOPPs)and its corresponding mechanism.METHODS The trophoblast cell line HTR-8/SV neo cells were cotreated with PZ(20μg·mL^(-1))and AOPPs(200μg·mL^(-1))for 48 h.The levels of cell cycle,senescence-associatedβ-galactosidase(SAβ-Gal),senescence-associated heterochromatin foci(SAHF)and mitochondrial membrane potential(ΔΨm)were measured to evaluate cellular senescence.The level of intracellular reactive oxygen species(ROS)was detected by flow cytometry,and the expression levels of proteins p53 and p21 were detected by Western blotting.RESULTS Co-treatment with AOPPs and PZ significantly deceased the percentages of cells in G0/G1 phase(P<0.01),and the levels of SAβ-Gal and SAHF(P<0.01 for both comparison)as well,and increased the percentages of cells in G2 phase(P<0.05)andΔΨm(P<0.01)compared with those in AOPPs group.The results of flow cytometry showed that co-treatment with AOPPs and PZ significantly decreased the level of ROS compared with that in AOPPs group(P<0.01).Meanwhile,co-treatment with AOPPs and PZ significantly down-regulated the relative expression levels of p53 and p21 proteins compared with those in AOPPs group(P<0.01 for both comparison).CONCLUSION PZ has antioxidant capacity and may reverse the pro-senescence effect of AOPPs on trophoblast cells.

关 键 词：晚期氧化蛋白产物 根皮苷 滋养细胞 衰老 活性氧 

分 类 号：R714.12[医药卫生—妇产科学]