三方益子方与3种不同补肾方对肾阳虚多尿模型大鼠的药效学研究  

Pharmacodynamic study of Sanfang Yizi Prescription and other three traditional kidney-tonifying prescriptions in rats with kidney-yang deficiency polyuria

作　　者：黄小洪 陈海燕 李芳婵 何耀莹 余昆莹 严森莹 林青华 蒋林 HUANG Xiaohong;CHEN Haiyan;LI Fangchan;HE Yaoying;YU Kunying;YAN Senying;LIN Qinghua;JIANG Lin(College of Pharmacy,Guangxi University of Traditional Chinese Medicine,Guangxi Nanning 530200,China;Guangxi Zhuang and Yao Medicine Engineering Technology Research Center,Guangxi Nanning 530200,China;Key Laboratory for TCM Extraction and Purification and Quality Analysis(Guangxi University of Chinese Medicine),Education Department of Guangxi Zhuang Autonomous Region,Guangxi Nanning 530200,China)

机构地区：[1]广西中医药大学药学院,广西南宁530200 [2]广西壮瑶药工程技术研究中心,广西南宁530200 [3]广西高校中药提取纯化与质量分析重点实验室,广西南宁530200

出　　处：《中国医院药学杂志》2023年第19期2154-2160,2178,共8页Chinese Journal of Hospital Pharmacy

基　　金：广西一流学科建设开放课题自然科学研究项目(编号:2019XK118);南宁市良庆区科学研究与技术开发计划项目(编号:201910)。

摘　　要：目的:比较三方益子方与不同补肾方干预腺嘌呤致肾阳虚多尿模型大鼠的药效学作用。方法:采用雄性SD大鼠灌胃腺嘌呤4周复制肾阳虚多尿大鼠模型,造模后2周开始同时给予三方益子方、缩泉丸、二精丸及水陆二仙丹药液,期间观察各组大鼠的日常行为,记录各组大鼠的夜尿和日尿,检测尿液中尿素氮和肌酐水平,末次给药后于腹主动脉取血,检测血浆中尿素氮、肌酐、醛固酮及抗利尿激素水平,称定肝脏、肾脏、脾脏、睾丸、肾上腺及膀胱质量并计算脏器指数,观察肾脏外观并HE染色观察肾脏组织病理学状态。结果:与空白组比较,模型组大鼠体质量增长缓慢,出现弓背蜷缩、皮毛暗淡稀疏、自主活动减少等表现,且夜尿量、24 h尿量、脏器指数、生化指标均具有显著性差异(P<0.05),表明肾阳虚多尿模型复制成功。给药4周后,与模型组比较,三方益子方组的夜尿量、24 h尿量均显著下降(P<0.05),其他各给药组的夜尿量、24 h尿量均显示差异无显著性(P>0.05);各给药组尿中尿素氮、肌酐水平均显著升高,血浆中尿素氮、肌酐显著下降,以及醛固酮、抗利尿激素水平均有显著上升(P<0.05),且仅三方益子方组血浆中各指标与空白组相比显示差异无显著性(P>0.05);各给药组大鼠的脏器指数、肾脏外观及肾脏HE切片结果具有不同程度的改善,其中三方益子方组改善效果最佳。结论:三方益子方对肾阳虚多尿模型大鼠具有显著的缩尿作用,其干预肾阳虚多尿模型大鼠的药效学作用优于缩泉丸、二精丸和水陆二仙丹。OBJECTIVE To compare the pharmacodynamic effects of Sanfang Yizi Prescription with different kidney-tonifying prescriptions in the adenine-induced kidney-yang deficiency polyuria model in rats.METHODS Rats with kidney-yang deficiency polyuria were induced by adenine intragastric administration for 4 weeks,and 2 weeks after modeling,the rats were respectively given Sanfang Yizi Prescription,Suoquanwan,Erjingwan,and Shuilu Erxiandan liquid.After treatment,the daily behavior of each group was observed,the nocturnal and daily urine of each group was recorded,and the levels of urea nitrogen and creatinine in urine were detected.The levels of urea nitrogen,creatinine,aldosterone and antidiuretic hormone were measured.The liver,kidney,spleen and testis were weighed and the organ indices were calculated.RESULTS Compared with those in the blank group,the rats in the model group showed slow weight gain,bowed back and curling up,dull and sparse fur,and reduced voluntary activities,and the nocturnal urine volume,daily urine volume,organ index and biochemical indexes were significantly different(P<0.05),indicating that the model of kidney-yang deficiency polyuria was successfully replicated.After 4 weeks of drug administration,compared with those in the model group,the nocturnal urine volume and daily urine volume in Sanfang Yizi Prescription group decreased significantly(P<0.05),and the effects were significantly better than those in the other drug groups,while the nocturnal urine volume and daily urine volume in the other drug groups showed no significant difference(P>0.05);the urinary level of urea nitrogen and creatinine in each drug group increased significantly(P<0.05).Compared with the blank group,there was no significant difference(P>0.05),among which the effects were most obvious in Sanfang Yizi Prescription group.The serum levels of urea nitrogen and creatinine decreased significantly and the levels of aldosterone and antidiuretic hormone increased significantly(P<0.05)in each drug administration group,and only the

关 键 词：三方益子方 缩泉丸 二精丸 水陆二仙丹 夜尿症 肾阳虚多尿模型 腺嘌呤 

分 类 号：R285.5[医药卫生—中药学]