A Dual Endogenous Stimuli-Responsive Framework Nucleic Acid Nanodevice for MicroRNA Imaging  

作　　者：Lie Li Haiyan Chen Xiaohong Wen Suping Li Mei Yang Qiuping Guo Kemin Wang 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Biology,College of Chemistry and Chemical Engineering,Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province,Hunan University,Changsha 410082

出　　处：《CCS Chemistry》2023年第10期2403-2414,共12页中国化学会会刊（英文）

基　　金：supported by the Natural Science Foundation of China(grant nos.21877030,21735002,and 21778016).

摘　　要：Framework nucleic acids(FNAs)have emerged as intelligent sensing systems for the detection of tumor-related biomarkers in living cells.However,orthogonally controlled manipulation of FNAs-based nanodevices for on-site imaging of microRNAs(miRNAs)remains an intractable challenge.Herein,we report a dual endogenous stimuli-responsive FNA nanodevice that can perform miRNA sensing and imaging in a tumor-specificmanner.The sensing function of the nanodevice is silent(OFF)by rationally incorporating adenosine 5′-triphosphate(ATP)aptamer and an abasic site,which can also be activated(ON)with ATP and human apurinic/apyrimidinic endonuclease 1(APE1)in tumor cells,enabling on-site and efficient miRNA imaging with improved tumor specificity.Furthermore,we demonstrate the capability of the nanodevice for specificmiRNAimagingboth in living cells and in vivo based on the“dual keys”(overexpressed ATP and APE1 in tumors)priming mode.Therefore,this work illustrates a simple biosensing methodology with great potential for precise imaging of tumor biomarkers in clinical diagnosis and therapeutic evaluation.

关 键 词：framework nucleic acids AND gate activatable biosensor fluorescence microRNA imaging 

分 类 号：TB34[一般工业技术—材料科学与工程]