穴位刺激联合COX-2抑制剂对乳腺癌骨转移大鼠镇痛、NK细胞活性及PK2蛋白水平的影响  

Effects of Acupoint Stimulation Combined with COX-2 Inhibitor on Analgesia,NK Cell Activity and PK2 Protein Level in Rats with Bone Metastasis of Breast Cancer

作　　者：马强 于珊珊 孟杰 马跃[1] 张华新 MA Qiang;YU Shanshan;MENG Jie;MA Yue;ZHANG Huaxin(Tangshan Union Medical College Hospital,Tangshan 063000,China)

机构地区：[1]唐山市协和医院,河北唐山063000

出　　处：《针灸临床杂志》2023年第9期80-86,共7页Journal of Clinical Acupuncture and Moxibustion

基　　金：河北省中医药类基金,编号:2022573。

摘　　要：目的:探究穴位刺激联合COX-2抑制剂对乳腺癌骨转移大鼠镇痛、NK细胞活性及PK2蛋白的作用机制。方法:选取50只SPF级SD雌性大鼠,随机数字表法分为正常(A)组、模型(B)组、穴位刺激(C)组、COX-2抑制剂(D)组与穴位刺激联合COX-2抑制剂(E)组,每组10只,对B、C、D与E组采用胫骨内注射乳腺癌细胞建立乳腺癌骨转移模型,A组不建立该模型,建模成功后,对C组给予穴位刺激治疗,对D组给予灌胃25 mg/kg的塞来昔布,对E组给予穴位刺激联合塞来昔布治疗,A组、B组同期给予灌胃同体积生理盐水,观察并记录大鼠疼痛情况,双能线骨密度仪检测大鼠骨密度及骨矿物质含量,HE染色法检测骨组织病理形态,免疫印迹法检测NK细胞活性,免疫组化法检测PK2蛋白表达。结果:与A组比较,B组50%PWT、TWL显著降低,差异具有统计学意义(P<0.05),双侧后肢负重差值显著升高,差异具有统计学意义(P<0.05),与B组比较,C组、D组与E组50%PWT、TWL明显升高,差异具有统计学意义(P<0.05),双侧后肢负重差值显著降低,差异具有统计学意义(P<0.05),且D组与C组比较差异无统计学意义(P>0.05),E组比D组变化明显,差异具有统计学意义(P<0.05);A组大鼠骨组织形态完整,骨小梁排列整齐,骨皮质连续,无坏死区域,骨髓腔内充满深染的血细胞,B组骨组织形态遭到破坏,骨皮质出现连续性中断,并可见大量肿瘤细胞浸润,瘤细胞形态不规则,且排列紊乱,细胞核大,异型性明显,与B组比较,C组、D组与E组病理形态明显改善;与A组比较,B组BMD、BMC显著降低,差异具有统计学意义(P<0.05),与B组比较,C组、D组、E组BMD、BMC显著升高,差异具有统计学意义(P<0.05),且D组与C组比较差异无统计学意义(P>0.05),E组比D组升高显著,差异具有统计学意义(P<0.05);与A组比较,B组大鼠胫骨组织中NKp30、NKp44、NKp46蛋白表达显著降低,差异具有统计学意义(P<0.05),与B组比较,C组、D组与E组大�Objective:To investigate the action mechanism of acupoint stimulation combined with COX-2 inhibitor on analgesia,NK cell activity and PK2 protein in treatment of bone metastases from breast cancer in rats.Methods:50 SPF SD female rats were randomly divided into the normal group(group A),the model group(group B),the acupoint stimulation group(group C),the COX-2 inhibitor group(group D),and the combination group(acupoint stimulation combined with COX-2 inhibitor,group E),with 10 rats in each group.The model of bone metastasis of breast cancer was established by injecting breast cancer cells into tibia.After successful modeling,group C was given acupoint stimulation therapy,group D was given Celecoxib(25 mg/kg)by gavage,and group E was given acupoint stimulation combined with Celecoxib;group A and group B were given the same volume of normal saline by gavage at the same time.Pain of rats was observed,bone mineral density and bone mineral content were detected by dual-energy line absorptiometry.Pathological morphology of bone tissues was detected by HE staining.NK cell activity was detected by Western blotting method.Expression of PK2 protein was assessed by immunohistochemistry.Results:Compared with those in group A,50%PWT and TWL were significantly decreased(P<0.05),and the difference in bilateral hindlimb weight-bearing was significantly increased in group B(P<0.05);compared with those in group B,50%PWT and TWL were significantly increased(P<0.05),and the difference in bilateral hindlimb weight-bearing was significantly decreased in group C,group D and group E(P<0.05);there were no statistical differences in 50%PWT,TWL and the difference in bilateral hindlimb weight-bearing between group C and group D(P>0.05);the changes in the above indexes were more significant in group E than those in group D(P<0.05).In group A,bone tissue morphology was complete,the trabecular bone was neatly arranged,the bone cortex was continuous without necrotic area,and the bone marrow cavity was full of hyper-stained blood cells;in group

关 键 词：穴位刺激 COX-2抑制剂 乳腺癌 骨转移 镇痛 NK细胞活性 PK2蛋白 

分 类 号：R246.5[医药卫生—针灸推拿学]