艾灸预处理对急性胃黏膜损伤大鼠NLRP3/Caspase-1/GSDMD通路的影响  

Effect of Moxibustion Preconditioning on NLRP3/Caspase-1/GSDMD Pathway in Rats with Acute Gastric Mucosal Injury

作　　者：阿斯卡尔•牙生 霍新慧[1] 阿力米热·阿布都热西提 张恒雷 马雨欣 张莉萍 ASKAR Yasheng;HUO Xinhui;ALIMIRE Abudurexiti;ZHANG Henglei;MA Yuxin;ZHANG Liping(Xinjiang Medical University,Urumqi 830001,China)

机构地区：[1]新疆医科大学,新疆乌鲁木齐830001

出　　处：《针灸临床杂志》2023年第10期76-81,共6页Journal of Clinical Acupuncture and Moxibustion

基　　金：新疆维吾尔自治区自然科学基金,编号:2021D01C224;国家级大学生创新课题,编号:202210760001;新疆医科大学第四附属医院院级重点课题,编号:ZYY2019ZD04。

摘　　要：目的:观察艾灸预处理对急性胃黏膜损伤大鼠胃黏膜细胞焦亡NLRP3/Caspase-1/GSDMD通路的影响。方法:将30只健康SPF级SD大鼠雌雄各半,分为正常组、模型组和艾灸预处理组。选取足三里(双侧)和中脘穴进行艾灸预处理7 d,再用无水乙醇灌胃法进行造模,观察胃黏膜组织形态,评估UI指数,ELISA法检测肿瘤坏死因子-α(TNF-α)、白介素1β(IL-1β)和白介素18(IL-18)3种细胞因子的表达量;Western blot检测大鼠胃组织炎性小体核苷酸寡聚化结构域样受体蛋白3(NLRP3)及胱天蛋白酶-1(Caspase-1)、消皮素(GSDMD)的表达水平。结果:艾灸预处理后,机体在应对致病因素刺激时可抑制IL-1β、IL-18及TNF-α的分泌,对比正常组和模型组均有明显下降(P<0.01),NLRP3、Caspase-1和GSDMD蛋白的表达量均低于正常组和模型组(P<0.01)。结论:艾灸预处理通过NLRP3/Caspase-1/GSDMD经典通路途径抑制细胞焦亡,降低炎症发生的程度,达到保护胃黏膜的作用。Objective:To observe the effect of moxibustion preconditioning on NLRP3/Caspase-1/GSDMD pathway of gastric mucosal cells in rats with acute gastric mucosal injury.Methods:30 healthy SPF SD rats were divided into the normal group,the model group and the moxibustion preconditioning group.Zusanli(ST36,bilateral)and Zhongwan(RN12)were selected for moxibustion preconditioning for 7 days,and then the acute gastric mucosal injury model was established by gastric perfusion with anhydrous ethanol.The morphology of gastric mucosa was observed,UI index was evaluated,ELISA method was used to detect the expressions of TNF-α,IL-1βand IL-18 in gastric tissues.Western blot method was used to detect the expressions of NLRP3,Caspase-1 and GSDMD in gastric tissues.Results:After moxibustion preconditioning,the body could inhibit the secretions of IL-1β,IL-18 and TNF-αin response to the stimulation of pathogenic factors,when compared with those in the normal group and the model group(P<0.01).The expressions of NLRP3,Caspase-1 and GSDMD were lower in the moxibustion preconditioning group than those in the normal group and the model group(P<0.01).Conclusion:Moxibustion preconditioning can inhibit pyroptosis through NLRP3/Caspase-1/GSDMD classical pathway,alleviate inflammatory response to achieve the role of protecting gastric mucosa.

关 键 词：艾灸预处理 急性胃黏膜损伤 细胞焦亡 NLRP3/Caspase-1/GSDMD 

分 类 号：R246.1[医药卫生—针灸推拿学]