Study on the mechanism of effect of Daodi Tongguan Decoction on rats with premature ovarian failure  

作　　者：LONG Yun-xia ZHONG Yi-hui WU Yuan-yuan CHEN Qiu-jin DU Li ZHENG Hong LI Yin-ying LUO Zhi-juan 

机构地区：[1]Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530011,China [2]Liuzhou Maternity and Child Healthcare Hospital,Liuzhou 545001,China

出　　处：《Journal of Hainan Medical University》2023年第12期18-23,共6页海南医学院学报(英文版)

基　　金：National Natural Science Foundation of China(No.81360594);Autonomous Region Traditional Chinese Medicine Inheritance and Development Special Fund Project‑Luo Zhijuan Guangxi Famous Traditional Chinese Medicine Inheritance Studio Construction Project(No.[2022]2)。

摘　　要：Objective:To investigate the mechanism of the effect of Daodi Tongguan Decoction on ovarian function in rats with premature ovarian failure.Methods:Forty SD female rats with regular estrous cycle were randomly divided into the blank group,the model group,the positive group(Bujiale 0.099 mg/kg),the Daodigitongguan decoction high-dose group(1.4 g/100 g)and the Daodigitongguan decoction medium-dose group(0.7 g/100 g).The model group,positive group,Daodi Tongguan Decoction high-dose group and Daodi Tongguan decoction medium-dose group were given Tripterygium wilfordii polyglycoside tablets suspension(50 mg/kg)by continuous gavage for 14 days to establish a rat model of premature ovarian failure.After the modeling,each group was given the corresponding intervention agent for 28 consecutive days.After the intervention,serum and ovarian tissue were collected from rats in each group.The morphological structure of rat ovarian tissue was observed after HE staining.Serum AMH level was determined by ELISA.The expressions of Bcl-2,Bax and VEGF in ovarian tissue were detected by IHC.The apoptosis of granulosa cells in ovary was observed by TUNEL.Results:Compared to the normal group,the growth follicles in the model group were reduced and the atretic follicles were increased.Serum AMH level was significantly decreased(P<0.01).The expression of Bax protein in ovarian tissue was increased(P<0.01).The protein expressions of Bcl-2 and VEGF were decreased(P<0.01,P<0.05),and the apoptosis rate of follicular granulosa cells in ovarian tissue was increased(P<0.01).Compared to the molding group,the serum AMH level in the TCM dose groups was increased to varying degrees,and the change of the AMH in high-dose group was the most significant(P<0.05).The expression of Bax protein in ovarian tissue decreased(P<0.05),while the expression of Bcl-2 and VEGF protein increased(P<0.05,P<0.05).The apoptosis rate of follicular granulosa cells in ovarian tissue was decreased in the high dose group(P<0.05).Conclusion:Daodi Tongguan Decoction can improv

关 键 词：LPremature ovarian failure Daodi Tong guan soup VEGF BAX Bcl-2 APOPTOSIS 

分 类 号：R711.75[医药卫生—妇产科学]