茄子查尔酮合酶基因的克隆与生物信息学分析  

Cloning and Bioinformatics Analysis of Chalcone Synthase Gene in Eggplant

作　　者：何金娇 张万方[1] 侯玥如 徐鑫[1] 毛雪飞[1] 吴云舟[2] HE Jinjiao;ZHANG Wanfang;HOU Yueru;XU Xin;MAO Xuefei;WU Yunzhou(School of Biological Engineering,Xinxiang University,Xinxiang,Henan 453003;College of Life Science,Northeast Agricultural University,Harbin,Heilongjiang 150030)

机构地区：[1]新乡学院生物工程学院,河南新乡453003 [2]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《北方园艺》2023年第19期16-21,共6页Northern Horticulture

基　　金：国家自然科学基金青年资助项目(31502050);河南省高等学校青年骨干教师培养计划资助项目(2019GGJS246);新乡学院博士科研启动资助项目(1366020118)。

摘　　要：以“杭茄1号”紫茄子苗为试材,采用RT-PCR方法克隆CHS基因,利用DNAMAN、MEGA 7.0.26及一些在线网站研究了CHS基因所编码蛋白的结构与功能,以期为研究查尔酮合酶CHS基因在茄子的生长发育中的意义及植物类黄酮的生物合成及其分子机制提供参考依据,同时丰富了双子叶植物CHS超家族的相关研究。结果表明:获得CHS基因全长为1170 bp,预测该基因编码的蛋白很有可能定位于细胞质中。系统进化分析表明,紫茄子与番茄、芒果、咖啡、短茄在同一个分支,亲缘关系最近,均属于茄科植物。‘Hang Eggplant No.1'was used as material,the CHS gene was cloned by RT-PCR,and the structure and function of the protein encoded by the CHS gene were analyzed by DNAMAN,MEGA 7.0.26 and some online websites,in order to provide reference for the study of the significance of chalcone synthase gene CHS in the growth and development of eggplant and the biosynthesis and molecular mechanism of plant flavonoids,and also enrich the related studies of CHS superfamily in dicotyledons.The results showed that the full length of CHS gene encoded protein was 1170 bp,and it was predicted that CHS gene might be located in cytoplasm.The phylogenetic analysis showed that aubergine eggplant,tomato,mango,coffee and nightshade were in the same branch,and they all belong to nightshade family.

关 键 词：紫茄子 查尔酮合酶 基因克隆 生物信息学分析 

分 类 号：S641.1[农业科学—蔬菜学]