盐胁迫下外源Ca^(2+)添加对藤本月季光合及氮代谢相关酶活性的调控  

Regulation of Exogenous Ca^(2+)Addition on the Activities of Enzymes Related to Photosynthesis and Nitrogen Metabolism in Climbing Rose Under Salt Stress

作　　者：李婕[1] 周凯 何文峰 LI Jie;ZHOU Kai;HE Wenfeng(College of Landscape Architecture,Shangqiu University,Shangqiu,Henan 476000;College of Agronomy,Henan Agricultural University,Zhengzhou,Henan 450003;Henan Yuyi Seed Technology Development Co.Ltd.,Zhengzhou,Henan 450000)

机构地区：[1]商丘学院风景园林与建筑学院,河南商丘476000 [2]河南农业大学农学院,河南郑州450003 [3]河南豫艺种业科技发展有限公司,河南郑州450000

出　　处：《北方园艺》2023年第19期57-65,共9页Northern Horticulture

基　　金：国家自然科学基金资助项目(32000170)。

摘　　要：以藤本月季“大游行”为试材,采用土壤喷施0、200 mmol·L^(-1)盐溶液的处理方法,探究不同浓度外源Ca^(2+)(0、5、10、20 mmol·L^(-1))对藤本月季光合以及氮代谢相关酶活性的影响,以期为外源Ca^(2+)缓解盐胁迫对藤本月季的伤害提供参考依据。结果表明:一定浓度外源Ca^(2+)能够增加藤本月季株高生长量、叶面积和地上生物量,促进光合作用,提高叶片中光合色素含量以及氮代谢相关酶活性来增强植物抗性。在200 mmol·L^(-1)盐处理下,与0 mmol·L^(-1)Ca^(2+)处理浓度相比,添加10 mmol·L^(-1)Ca^(2+)浓度处理下藤本月季的叶绿素a、叶绿素b、总叶绿素(a+b)以及叶黄素含量分别增加了143.9%、152.5%、151.9%和90.6%;光合参数中水分利用效率(WUE)、净光合速率(P_(n))、蒸腾速率(T_(r))、胞间CO_(2)浓度(C_(i))和气孔导度(G_(s))分别增加96.6%、60.5%、353.3%、43.0%和80.6%,气孔限制值(Ls)降低63.8%,PSⅡ最大光化学效率(F_(v)/F_(m))和PSⅡ潜在活性(F_(v)/F_(o))显著增加;此外,盐胁迫下,藤本月季叶片中硝态氮含量下降,铵态氮含量增加,一定浓度外源Ca^(2+)处理能显著增加硝酸还原酶(NR)、谷氨酰胺合成酶(GS)以及异柠檬酸脱氢酶(NADP-ICDH)活性,而降低谷氨酸脱氢酶(NADH-GDH)活性。综上可知,添加一定量的Ca^(2+)能增加藤本月季光合色素和硝态氮含量,促进氮代谢过程中相关酶活性增加,提高植物光合作用,进一步促进植物生长来抵御盐胁迫,其中以添加10 mmol·L^(-1)Ca^(2+)处理效果最好。Taking climbing rose Parade'as test material,the effects of different concentrations of exogenous calcium(0,5,10,20 mmol·L^(-1))on photosynthesis and nitrogen metabolism related enzyme activity in climbing roses were studied by spraying O,200 mmol.L^(-1)salt solution onto the soils,in order to provide reference for exogenous Ca^(2+)to alleviate the damage of salt stress to climbing rose.The results showed that a certain concentration of exogenous Ca^(2+)could increase the height growth,leaf area,and aboveground biomass of climbing rose,promote photosynthesis,increase the content of photosynthetic pigments in leaves,and enhance the activity of nitrogen metabolism related enzymes to enhance plant resistance Under 200 mmol·L^(-1)salt treatment,compared with O mmol·L^(-1)Ca^(2+)treatment concentration,adding 10 mmol·L^(-1)Ca^(2+)concentration increased the chlorophyll a,chlorophyll b,total chlorophyll(a+b),and lutein content of climbing roses by 143.9%,152.5%,151.9%and 90.6%,respectively.The photosynthetic parameters of water use efficiency(WUE),net photosynthetic rate(P_(n)),transpiration rate(T_(r)),intercellular CO_(2)concentration(C_(i))and stomatal conductance(G_(s))increased by 96.6%,60.5%,353.3%,43.0%and 80.6%,respectively.The Ls decreased by 63.8%,and the maximum photochemical efficiency(F_(v)/F_(m))and potential activity(F_(v)/F_(o))of PS II significantly increased.In addition,under salt stress,the nitrate nitrogen content in the leaves of climbing rose decreased,while the ammonium nitrogen content increased.A certain concentration of exogenous Ca^(2+)treatment could significantly increase the activities of nitrate reductase(NR),glutamine synthetase(GS)and isocitrate dehydrogenase(NADP-ICDH),while reducing the activity of glutamine dehydrogenase(NADH-GDH).The conclusion was that adding a certain amount of Ca^(2+)could increase the photosynthetic pigments and nitrate nitrogen content of climbing rose,promoted the activity of related enzymes in nitrogen metabolism,improved plant photosynthesis,and further

关 键 词：盐 外源Ca^(2+) 藤本月季 光合作用 氮代谢 

分 类 号：S685.12[农业科学—观赏园艺]