纤维素降解细菌的筛选、鉴定及产酶条件优化  

Screening,identification and enzyme-producing conditions optimization of cellulose-degrading bacterial strains

作　　者：彭永妍 杨彬彬 李志羲 何玲 伍勇 邱成书 PENG Yongyan;YANG Binbin;LI Zhixi;HE Ling;WU Yong;QIU Chengshu(College of Chemistry and Life Sciences,Chengdu Normal University,Chengdu 611130,China)

机构地区：[1]成都师范学院化学与生命科学学院,四川成都611130

出　　处：《贵州科学》2023年第4期41-45,共5页Guizhou Science

基　　金：大学生创新创业项目(S202014389167)。

摘　　要：在成都师范学院校园采集土壤,经过两次富集培养。选择羧甲基纤维素钠-刚果红平板结合降解圈直径和菌落直径的比值进行初筛,筛选得到两株生长良好的菌株,编号S-04和S-10。利用液体产酶培养基,测定羧甲基纤维素酶和滤纸酶的活性进行复筛。综合两种酶活性,最终筛选出纤维素降解效果较好的菌株S-04。以S-04菌株为研究对象,研究其形态观察、革兰氏染色、生理生化鉴定、分子鉴定,并对其产酶条件优化。实验结果显示:S-04号菌株菌落为圆形,表面平滑,边缘较平整,中央较突出,较湿润,菌落整体不透明,呈现淡黄色,革兰氏阴性菌。结合生理生化和分子鉴定,该菌为产碱杆菌属中的粪产碱菌(Alcaligenes faecalis)。该菌产酶最适碳源为羧甲基纤维素钠(CMC-Na);最适氮源为牛肉膏。Soil samples were collected from the campus of Chengdu Normal University.Two strains were screened out from the collected samples by the carboxymethyl cellulose-Congo red medium and the ratio of the diameter of the degradation circle to the colony diameter,and were named S-04 and S-10.Strain S-04 was selected by detecting the activities of carboxymethyl cellulase(CMCase) and filter paper enzyme.Strain S-04 was determined to be Alcaligenes faecalis based on morphologic observation,Gram staining,physiological-biochemical identification,and molecular identification.The optimum enzyme-producing conditions for S-04 were as follows:CMC-Na as the carbon source,and beef extract as the medium.

关 键 词：纤维素降解细菌 筛选 鉴定 羧甲基纤维素酶 滤纸酶 

分 类 号：Q939.96[生物学—微生物学]