序列特异引物引导的聚合酶链式反应(PCR-SSP)结合血清学在疑难血型鉴定中的应用  

The application of PCR-SSP with the serology in identification and genotyping of ABO ambiguous blood group

作　　者：宋艳艳 张羽茜 曹昕瑞 于笑难 郑伟 SONG Yanyan;ZHANG Yuxi;CAO Xinrui;YU Xiaonan;ZHENG Wei(Department of Blood Transfusion of General Hospital of Northern Theater Command,Shenyang 110016,China)

机构地区：[1]中国人民解放军北部战区总医院输血医学科,辽宁沈阳110016

出　　处：《细胞与分子免疫学杂志》2023年第9期824-827,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：辽宁省应用基础研究计划联合计划项目(2022JH2/101500018)。

摘　　要：目的 探讨血型血清学与序列特异引物引导的聚合酶链式反应(PCR-SSP)基因分型在ABO疑难血型鉴定中的作用。方法 用血清学分析鉴定的ABO疑难血型标本80例,同时采用PCR-SSP法进行基因分型,结合两种方法学结果,确定血型及制定输血策略。结果 血清学鉴定亚型40例,其他原因引起的抗原抗体缺失或减弱正常血型40例;PCR-SSP法基因分型亚型41例(3例二者结果不一致:血清学Ael型1例,基因分型O2O2;血清学O型1例,基因分型BO1;血清学A型1例,基因分型AB),正常血型39例。结论 使用血清学结合基因分型鉴定ABO疑难血型具有重要意义。Objective To investigate the effect of blood group serology and polymerase chain reaction with sequence-specific primers(PCR-SSP)on identification and genotyping of ambiguous ABO blood group.Methods Eighty suspicious ABO blood group samples were identified by serology and polymerase chain reaction with sequence-specific primers(PCR-SSP).The final blood group type and the strategy of the transfusion of each case were determined according to the results of serology and PCR-SSP.Results 40 cases were confirmed to be subtypes,and the remaining 40 cases were normal types with weakened antigens or missing antibodies due to other reasons.The results of molecular genetic blood group typing based on PCR-SSP were 41 cases of subtypes(There were 3 discrepancies between two methods:one was Ael identified by serological methods,while its gene type was O0202;one was common type O,while its gene type was BO1;one was type A,while its gene type was AB.)and 39 cases of normal ones.Conclusion Genotyping technology combined with serological typing has an important significance in identification of ABO blood groups.

关 键 词：ABO血型 血型血清学 序列特异引物引导的聚合酶链式反应(PCR-SSP) 疑难血型 

分 类 号：R457.1[医药卫生—治疗学] R722.19[医药卫生—临床医学]