伏立康唑与两性霉素B对土曲霉和黑曲霉的抗菌活性比较  

Comparison of Antifungal Activity of Voriconazole and Amphotericin B against Aspergillus Terreus and Aspergillus Niger

作　　者：薛阳 张薇 李红磊 邢学锋[2] 胡燕君 XUE Yang;ZHANG Wei;LI Hong-lei;XING Xue-feng;HU Yan-jun(Beijing South Medical District of Chinese PLA General Hospital,Beijing 100072,China;School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]解放军总医院京南医疗区,北京100072 [2]南方医科大学中医药学院,广东广州510515

出　　处：《解放军药学学报》2023年第5期445-448,共4页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的比较伏立康唑和两性霉素B对曲霉菌的抗菌活性以及量效关系。方法根据美国临床实验室标准化协会M38-A活化黑曲霉和土曲霉菌株并制备菌液,做伏立康唑和两性霉素B在体外对黑曲霉和土曲霉最低抑菌浓度测试,进行量效关系实验、统计分析。结果在受试曲霉菌株中,伏立康唑对土曲霉的最低抑菌浓度为0.49μg·ml-1,两性霉素B对土曲霉的最低抑菌浓度为7.8μg·ml-1。量效关系结果显示,在测试的48h内,伏立康唑对土曲霉呈现强的抑制作用。结论伏立康唑对黑曲霉和土曲霉呈现较好抗菌效能,两性霉素B对土曲霉显现出较低的抗菌效能。Objective To compare the antifungal activity of voriconazole and Amphotericin B against Aspergillus spp,and study their dose-effect relationship.Methods Aspergillus niger and Aspergillus terreus strains were activated,whose fungal liquid was prepared according to Clinical Laboratory Standards M38-A.The minimum inhibitory concentrations of voriconazole and Amphotericin B against the above fungal strains were tested in vitro.The results of statistical analysis were also compared.Results The results showed that the MIC value of voriconazole against Aspergillus terreus was 0.49μg·ml-1 while the MIC value of Amphotericin B was 7.8μg·ml-1.In addition,the study on dose-effect relationship of antifungal activity revealed that voriconazole could inhibit the growth of Aspergillus terreus in 48 hours.Conclusion Voriconazole exhibits a good antifungal activity on Aspergillus spp.,while Amphotericin B is insensitive to Aspergillus terreus.

关 键 词：伏立康唑 两性霉素B 曲霉菌 

分 类 号：R978.5[医药卫生—药品]