外周血固有淋巴样细胞(ILC)平衡失调维持免疫抑制状态促进肺腺癌发展  

Imbalance of lymphoid cells in peripheral blood maintains the immunosuppression and promotes the development of lung adenocarcinoma

作　　者：郭会 臧利超 杨歆煜 吴玉敏 马锦洪[1] 史伟峰[1] GUO Hui;ZANG Lichao;YANG Xinyu;WU Yumin;MA Jinhong;SHI Weifeng(Department of Laboratory Medicine,The Third Affiliated Hospital of Soochow University,Changzhou 213003,China)

机构地区：[1]苏州大学附属第三医院检验科,江苏常州213003

出　　处：《细胞与分子免疫学杂志》2023年第8期729-736,共8页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81801568,81572052)。

摘　　要：目的 探讨肺腺癌患者外周血中固有淋巴样细胞(ILC)平衡失调后对下游单核巨噬细胞、辅助性T(Th)细胞平衡状态的影响,明确ILC平衡失调后对肺腺癌免疫状态以及转归的影响。方法 收集20例肺腺癌患者及正常体检者外周血,通过流式细胞术分析外周血中ILC、单核巨噬细胞及T淋巴细胞的百分比,并采用实时荧光定量PCR检测外周血中各型免疫细胞特征性的细胞因子分泌水平。结果 与正常体检者相比,肺腺癌患者M2型单核巨噬细胞、1型固有淋巴样细胞(ILC1)与ILC2比例上调,M1型单核巨噬细胞、CD4^(+)T淋巴细胞与CD8^(+)T淋巴细胞比例下调;M1型单核巨噬细胞与ILC1相关细胞因子mRNA表达下调,而M2型单核巨噬细胞与ILC2相关细胞因子mRNA表达上调;CD4^(+)T细胞相关细胞因子T细胞表达的T盒(T-bet) mRNA表达下调;CD4^(+)T细胞相关细胞因子GATA结合蛋白3(GATA3) mRNA表达上调;CD8^(+)T细胞的程序性死亡蛋白1(PD-1)表达上调。结论 肺腺癌患者外周血中ILC平衡失调促使单核巨噬细胞与Th细胞平衡失调,三者共同维持了肺腺癌患者的免疫抑制状态,从而促进肺腺癌发展。Objectives Objectives To investigate how the imbalance of innate lymphoid cells(ILCs)in the peripheral blood of patients with lung adenocarcinoma affects the balance of downstream mononuclear macrophages and T helper(Th)cells,and to identify the impact of the imbalance of ILCs on the immune status and prognosis of lung adenocarcinoma.Methods The peripheral blood of 20 patients with lung adenocarcinoma and normal controls were collected.The percentage of ILCs,mononuclear macrophages and T lymphocyte in peripheral blood were analyzed by flow cytometry.The characteristic cytokine secretion levels of various types of immune cells in peripheral blood were detected by real-time fluorescence quantitative PCR.Results Compared with the normal controls,the proportion of M2 mononuclear macrophages,ILC1 and ILC2 in patients with lung adenocarcinoma was up-regulated,while the proportion of M1 mononuclear macrophages,CD4^(+)T and CD8^(+)T was down-regulated.The mRNA expression of related cytokines of M1 mononuclear macrophages and ILC1 were decreased;while the mRNA expression of related cytokines of M2 mononuclear macrophages and ILC2 were increased.Along with the decreased CD4^(+)T cells-associated cytokine T-bet mRNA expression,and the increased GATA3 mRNA expression.Moreover,the expression of PD-1 in CD8^(+)T cells was also up-regulated.Conclusion The imbalance of ILCs in peripheral blood of patients with lung adenocarcinoma promotes the imbalance of mononuclear macrophages and Th cells,which altogether maintains the immunosuppression in patients with lung adenocarcinoma,and promotes the development of lung adenocarcinoma.

关 键 词：肺腺癌 固有淋巴样细胞(ILC) 单核巨噬细胞 辅助性T细胞 

分 类 号：R392.12[医药卫生—免疫学] R563[医药卫生—基础医学] R734.2R364.5