高效液相色谱法测定甜梦胶囊中4种成分  

Simultaneous Determination of Four Components in Tianmeng Capsules by HPLC

作　　者：李志娇 巩长芹 张晓博 陈静 方君卉 杨小薛 LI Zhi-jiao;GONG Chang-qin;ZHANG Xiao-bo;CHEN Jing;FANG Jun-hui;YANG Xiao-xue(Linyi Inspection and Testing Center,Linyi 276001,China;Qilu Pharmaceutical Co.,Ltd.,Jinan 250000,China;Dong’e Ejiao Health Chain Co.,Ltd Linyi 276001,China;Zaozhuang Institute for Food and Drug Control,Zaozhuang 277102,China)

机构地区：[1]临沂市检验检测中心,山东临沂276001 [2]齐鲁制药有限公司,山东济南250000 [3]东阿阿胶健康连锁有限公司,山东临沂276001 [4]枣庄市食品药品检验检测中心,山东枣庄277102

出　　处：《食品与药品》2023年第5期453-456,共4页Food and Drug

摘　　要：目的建立高效液相色谱-波长切换法同时测定甜梦胶囊中毛蕊异黄酮葡萄糖苷、淫羊藿苷、朝藿定C、橙皮苷的含量。方法以Thermo Hypersil Gold AQ为色谱柱,选用乙腈(A)-0.2%甲酸(B)为流动相,梯度洗脱;毛蕊异黄酮葡萄糖苷检测波长为260 nm,淫羊藿苷、朝藿定C检测波长为270 nm,橙皮苷检测波长为283 nm;流速为1.0 ml/min,柱温为30℃。结果毛蕊异黄酮葡萄糖苷进样量在0.0406~0.6893μg(r=0.9996)、橙皮苷进样量在0.0607~0.8415μg(r=0.9999)、朝藿定C进样量在0.0354~0.5415μg(r=0.9999)、淫羊藿苷进样量在0.0262~0.3939μg(r=0.9999)范围内有良好的线性关系;平均回收率分别为98.86%,98.29%,98.87%,99.12%,RSD均在2.0%以内。结论此法准确、可靠、分离度高,重复性较好,可为甜梦胶囊的质量标准提供理论依据。Objective To establish a high performance liquid chromatography(HPLC)-wavelength switching method for the simultaneous determination of calycosin glucoside,icariin,epimedin C and hesperidin in Tianmeng Capsules.Methods The chromatographic separation was carried out on a Thermo hypersil gold AQ,using acetonitrile(A)-0.2%formic acid(B)as the mobile phase of gradient elution.The UV detection wavelengths of calycosin glucoside,icariin,epimedin C and hesperidin were set at 260,270,270 and 283 nm.The flow rate was 1.0 ml/min and the column temperature was set at 30℃.Results There was a good linear relationship in the range of 0.0406-0.6893μg(r=0.9996)for calycosin glucoside,0.0607-0.8415μg(r=0.9999)for hesperidin,0.0354-0.5415μg(r=0.9999)for epimedium C and 0.0262-0.3939μg(r=0.9999)for icariin.The average recoveries were 98.86%,98.29%,98.87%and 99.12%,respectively,with RSDs less than 2.0%.Conclusion The method is accurate,reliable,with high separation and good repeatability,thus can provide theoretical basis for the quality standard of Tianmeng Capsules.

关 键 词：甜梦胶囊 毛蕊异黄酮葡萄糖苷 淫羊藿苷 朝藿定C 橙皮苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]