Catalase promotes whitefly adaptation to high temperature by eliminating reactive oxygen species  

作　　者：Peng Liang Jie Ning Wenlu Wang Pu Zhu Lianyou Gui Wen Xie Youjun Zhang 

机构地区：[1]Forewarning and Management of Agricultural and Forestry Pests,Hubei Engineering Technology Center,Institute of Insect,College of Agriculture,Yangtze University,Jingzhou,Hubei Province,China [2]Department of Plant Protection,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing,China [3]Jinhua Academy of Agricultural Sciences,Jinhua,Zhejiang,China

出　　处：《Insect Science》2023年第5期1293-1308,共16页昆虫科学(英文版)

基　　金：supported by grants from the National Natural Science Foundation of China(31871970,32221004,and 32272534);the Hainan Major Science and Technology Project(ZDKJ2021007);the Project of Sanya Yazhou Bay Science and Technology City(SKJC-2022-PTDX-017);the Research and demonstration of key technologies for ensuring supply of vegetable industry(2020-J-005-ydhz).

摘　　要：Thermal stress usually leads to excessive production of reactive oxygen species(ROS)in all aerobic organisms.Catalases(CAT)are the key antioxidant enzymes,which act as the first line of defense against ROS in the antioxidant pathway.The highly invasive and widely distributed whitefly Bemisia tabaci MED damages plants by feeding as well as by transmitting many plant viruses.Previous studies have shown that strong adaptability to high temperature helps explain the spread of MED around the world.However,the mechanism underlying high temperature adaptation of this pest is not well understood.In this study,6 CAT genes were identified from the MED genome and transcriptome dataset,among which BtCAT1,BtCAT2,and BtCAT3 were found to be highly expressed in adults.The expression of BtCAT1,BtCAT2,or BtCAT3 increased with induction temperature and induction time.The MED was exposed with mean high temperature(30℃ or 35℃)and a short-term extremely high temperature(39℃ or 41℃)after the silencing of BtCAT1,BtCAT2,or BtCAT3 to significantly increased ROS levels by at least 0.5 times and significantly decreased survival rate and fecundity of MED adults.The ROS level in the treated specimens gradually returned to a normal level after 24 h at 25℃,but the survival rate still declined significantly.Taken together,our results demonstrate that CAT could help B.tabaci adapt to long-term mean high temperatures and short-term extremely high temperatures by eliminating excessive ROS.

关 键 词：CATALASE FECUNDITY reactive oxygen species survival rate temperature adap-tation mechanism WHITEFLY 

分 类 号：Q96[生物学—昆虫学]