GARP: A family of glycine and alanine-rich proteins that helps spider mites feed on plants  

作　　者：Qin-Zhe Sun Xiao-Lin Li Yu-Fei Shi Yan-Chun Zhang Wen-Jie Chai Ruo-Yu Chen Jinzhi Niu Jin-Jun Wang 

机构地区：[1]Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Academy of Agricultural Sciences,Southwest University,Chongqing,China

出　　处：《Insect Science》2023年第5期1337-1351,共15页昆虫科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(32272525);the Chongqing Talent Program(cstc2022ycjh-bgzxm0008);the China Agriculture Research System(CARS-26);[Correction added on 27 Feb 2023,after first online publication:The funding number for National Natural Science Foundation of China has been changed from 322725535to32272525.]。

摘　　要：Spider mites(Tetranychidae)are destructive agricultural pests which have evolved strategies to overcome plant defenses,such as the ability to puncture the leaves of their hosts to feed.The expression of many genes with unknown functions is altered during feeding,but little is known about the role of these genes in plant–mite interactions.Here,we identified 3 novel gene families through analysis of genomic and transcriptomic data from 3 spider mite species.These GARP family genes encode glycine and alanine-rich proteins;they are present in mites(Acariformes)but absent in ticks(Parasitiformes)in the subclass Acari,indicating that these genes have undergone a significant expansion in spider mites and thus play important adaptive roles.Transcriptomic analysis revealed that the expression of GARP genes is strongly correlated with feeding and the transfer to new hosts.We used RNA interference to silence GARP1d in the two-spotted spider mite Tetranychus urticae,which inhibited feeding and egg laying and significantly increased mortality when the mites were transferred to soybean shoots;a similar effect was observed after TuVATPase was silenced.However,no changes in mite mortality were observed after TuGARP1d-silenced mites were placed on an artificial diet,which was different from the effect of TuVATPase silencing.Our results indicate that GARP family members play important roles in mite–plant interactions.Additional studies are needed to clarify the mechanisms underlying these interactions.

关 键 词：feeding mite-plant interaction RNA interference Tetranychus urticae Tetranychus evansi 

分 类 号：Q96[生物学—昆虫学]