Ligand dose-dependent activation of signaling pathways through the gustatory receptor NlGr11 linked to feeding efficacy in Nilaparvata lugens  

作　　者：Wei-Wen Chen Kai Lin Jun Lv Qin Su Meng-Yi Zhang Kui Kang Wen-Qing Zhang 

机构地区：[1]State Key Laboratory of Biocontrol and School of Life Sciences,Sun Yat-sen University,Guangzhou,China [2]College of Biology and Agriculture,Zunyi Normal University,Zunyi,Guizhou Province,China

出　　处：《Insect Science》2023年第5期1420-1430,共11页昆虫科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(31730073,U1401212);Guizhou Science and Technology Project(qiankeheji-zk[2021]General 135).

摘　　要：Insects often face both conditions with sufficient nutrients and conditions of undernutrition in the field.Through gustatory receptors,insects sense nutrients and regulate their physiological functions such as feeding and reproduction.However,it remains unclear whether signaling pathways activated by gustatory receptors depend on the concentration of nutrients and whether the difference in signaling pathways directly affects insects’physiological functions.Herein,we found that a sugar gustatory receptor,NlGr11,from the brown planthopper(BPH),Nilaparvata lugens,activated G protein-coupled signaling and ionotropic pathways when bound to high galactose concentration.BPHs subsequently demonstrated longer feeding times,feeding loads,and higher vitellogenin(NlVg)expression than BPHs exposed to high galactose concentrations,which only activated the ionotropic pathway.For the first time,our findings link plant nutrient conditions,signaling pathways activated by nutrients,and their gustatory receptors,and nutrient dose-dependent feeding efficacy and vitellogenin(Vg)expression in an insect.This will help us to better understand the molecular mechanism for insect feeding strategies on plants at different stages of nutritional conditions.

关 键 词：feeding efficacy galactose G protein-coupled signaling pathway insect gustatory receptor ionotropic pathway 

分 类 号：Q96[生物学—昆虫学]