Pheromones emitted by both female and male moths regulate coordination between the sexes for Agriphila aeneociliella (Lepidoptera: Crambidae)  

作　　者：Yi-Di Zhan Ying-Jie Liu Jia-Hui Liu Yong Liu 

机构地区：[1]College of Plant Protection,Shandong Agricultural University,Taian,Shandong Province,China [2]Staff Development Institute of China National Tobacco Corporation,Zhengzhou,China [3]Functional and Evolutionary Entomology,Gembloux Agro-Bio Tech,Liège University,Gembloux,Belgium

出　　处：《Insect Science》2023年第5期1481-1492,共12页昆虫科学(英文版)

基　　金：supported by grants from the Natural Science Foundation of Shandong Province(ZR2020MC121);the National Key R&D Program of China(2017YFD0201705).

摘　　要：The complex and efficient sex pheromone communication system in insects is essential for reproduction and for reproductive isolation of species.In moths,sex pheromone communication starts with male attraction to compounds emitted by females;only a few species act in the reverse.However,how the pheromones that are emitted by both sexes co-regulate and coordinate mate finding and mating remains unknown.Here,we identified both the male and female pheromones of Eastern Grass Veneer moth,Agriphila aeneociliella(Lepidoptera:Crambidae),and demonstrated their efficiency in manipulating behavioral responses of the opposite sex.Combining data from analysis of gas chromatography-electroantennogram detection,gas chromatography-mass spectrometry,and olfactory behavior assays,the female pheromone of A.aeneociliella was identified as(Z,Z,Z)-9,12,15-octadecatrienal and(Z)-9-hexadecenyl acetate,while the male pheromone was determined to be 1-nonanal.Both the 2 individual components of the female pheromone and their binary mixture were significantly attractive to males,and the 1-nonanal male pheromone induced strong electrophysiological responses in females and induced attraction of females in a Y-tube olfactory test.Depending on the concentration of 1-nonanal,its addition to the binary mixture of the female pheromone either enhanced(10^(−3) or 10^(−2)μg/μL)or reduced(1μg/μL)the aphrodisiac effect of the mixture on males.In wind-tunnel bioassays,different concentrations of pheromones,including the binary mixture of female pheromone and the mixture of male and female pheromones,had significant effects on male behavior.Our findings suggested that the blend of both female and male pheromones plays a significant role in the sexual communication system in some moths.

关 键 词：CRAMBIDAE male pheromone reproductive strategy sexual reproduction wheat pest 

分 类 号：Q96[生物学—昆虫学]