Expression and functional characterization of odorant-binding protein 2 in the predatory mite Neoseiulus barkeri  

作　　者：Yixia Wu Yaying Li Wenqiang Chu Tiandi Niu Xiaotian Feng Rongjiang Ma Huai Liu 

机构地区：[1]Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Beibei District,Chongqing,400715 China

出　　处：《Insect Science》2023年第5期1493-1506,共14页昆虫科学(英文版)

基　　金：This research was funded by the National Key R&D Program of China(2021YFC2600100);the National Natural Science Foundation of China(32072483,31901944);the Natural Science Foundation of Chongqing,China(cstc2020jcyj-msxmX0407);the Technology Innovation and Application Development Foundation of Chongqing,China(cstc2021jscx-lyjsAX0005);the Fundamental Research Funds for the Central Universities(SWUKQ22019).

摘　　要：Olfaction plays a crucial role for arthropods in foraging,mating,and oviposition.The odorant-binding protein(OBP)gene is considered one of the most important olfactory genes.However,little is known about its functions in predatory mites.Here,we used Neoseiulus barkeri,an important commercialized natural pest control,to explore the chemosensory characteristics of OBP.In this study,N.barkeri was attracted by methyl salicylate(MeSA)and showed higher crawling speeds under MeSA treatment.Then,we identified and cloned an OBP gene named Nbarobp2 and analyzed its expression profiles in the predatory mite.Nbarobp2 was 663 bp,was highly expressed in larval and nymphal stages,and was significantly upregulated in N.barkeri under MeSA treatment.Nbarobp2 encoded 202 amino acid residues with a molecular weight of 23 kDa(after removing the signal peptide).Sequence comparisons revealed that the OBPs in Arachnida shared 6 conserved cysteine sites,but were distinguishable from the OBPs of Insecta on the phylogenetic tree.RNA interference,Western blotting,and binding affinity assays further proved that Nbarobp2 was involved in volatile perception in predatory mites.This study shed light on the functional characteristics of OBPs in predatory mites,providing a new insight for better biological control.

关 键 词：binding affinity crawling speed heterologous expression methyl salicylate olfactory behavior RNA interference 

分 类 号：Q96[生物学—昆虫学]