Metabolomics of astaxanthin hyperaccumulation in Haematococcus pluvialis under high light stress  

作　　者：Yong DOU Jiayi LI Wenli ZHOU 

机构地区：[1]Tianjin Agricultural University,College of Fisheries,Tianjin Key Lab for Aquaculture Ecology and Cultivation,Tianjin 300384,China [2]Tianjin Modern Tianjiao Agricultural Technology Co.,Ltd.,Tianjin Key Laboratory for Green and Ecological Forage,Tianjin 301800,China

出　　处：《Journal of Oceanology and Limnology》2023年第5期1876-1886,共11页海洋湖沼学报（英文）

基　　金：Supported by the Tianjin Excellent Science and Technology Commissioners Project (No.22ZYCGSN00010);the Open Fund of Tianjin Key Laboratory of Aquatic Ecology and Aquaculture (No.TJAE201805);the Open Fund of Key Laboratory of Marine Ecosystem Dynamics (No.MED202013);the Tianjin Natural Science Foundation Project (No.18JCQNJC14800)。

摘　　要：Variation in metabolite profiles of Haematococcus pluvialis(a type of unicellular green algal)under light stress is a key issue of study at the present.To investigate the effect of light intensity on accumulation of astaxanthin in H.pluvialis,a 26-day batch culture experiment of H.pluvialis under the light intensity levels at 73,127,182,236,and 291μmol/(m^(2)·s)was conducted.Therefore,the optimal light intensity and the corresponding metabolic pathways of accumulation in H.pluvialis were determined.Results show that 236μmol/(m^(2)·s)was the optimum light intensity to induce astaxanthin accumulation,at which a maximum content of 9.01 mg/L was achieved on Day 24.A total of 132 metabolites were identified and quantified,of which 38 differential metabolites were highlighted and classified,including 3 fatty acids or intermediates,5 amino acids or derivatives,5 carbohydrates or intermediates,16nucleoside derivatives,and 9 other metabolites using LC-MS/MS technique.Subsequently,16 statistically significant differential metabolic pathways were enriched and annotated based on Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis between the control and the 236μmol/(m^(2)·s)treatment group(P<0.05).In addition,the bioprocesses included cellular basal metabolism and signaling systems,such as carbohydrate metabolism,amino acid metabolism,glycerol and derivatives metabolism,nucleotide and derivative metabolism,and inositol phosphate metabolism were activated and regulated under strong light stress conditions.Moreover,4 hub metabolites containing D-glucose-6-phosphate,L-tyrosine,glycerol-3-phosphate,and L-glutamine were identified,based on which the associated metabolic network was constructed.The study provided a metabolomic view of astaxanthin accumulation in H.pluvialis under strong light stress.

关 键 词：Haematococcus pluvialis ASTAXANTHIN high light stress metabolomic analysis 

分 类 号：Q945.78[生物学—植物学]