祛瘀清热方对尿酸钠诱导的痛风性关节炎大鼠NLRP6/Caspase-1/IL-1β信号通路的影响  

Effect of Quyu Qingre Prescription on NLRP6/Caspase-1/IL-1β Signaling Pathway in Rats with Gouty Arthritis Induced by Sodium Urate

作　　者：钟秋生 杨巧玉 邵洁琦 黄欢欢 陈博 王昱 ZHONG Qiusheng;YANG Qiaoyu;SHAO Jieqi;HUANG Huanhuan;CHEN Bo;WANG Yu(Songshan Lake Central Hospital,Dongguan Guangdong 523326,China)

机构地区：[1]东莞市松山湖中心医院,广东东莞523326

出　　处：《新中医》2023年第18期1-5,共5页New Chinese Medicine

基　　金：广东省中医药局科研课题(20221417)。

摘　　要：目的:观察祛瘀清热方对尿酸钠诱导的痛风性关节炎(GA)大鼠NLRP6/Caspase-1/IL-1β信号通路的影响。方法:检测受试大鼠踝关节肿胀度。用酶联免疫吸附法(ELISA)测定大鼠血清白细胞介素-1β (IL-1β)、肿瘤坏死因子-α (TNF-α)的含量,用Western Blot检测滑膜组织NLRP6和Caspase-1含量。结果:与正常组比较,其余各组造模之后,在不同时间点上,受试大鼠踝关节肿胀度增加(P<0.05)。与模型组比较,祛瘀清热方低、中、高剂量组及秋水仙碱组大鼠,在造模后各时间节点,大鼠踝关节肿胀度降低(P<0.05)。与秋水仙碱组比较,祛瘀清热方低、中、高剂量组受试大鼠,在造模后各时间节点,大鼠踝关节肿胀度增加(P<0.05)。模型组大鼠血清IL-1β、TNF-α水平较正常组上升(P<0.05)。与模型组比较,祛瘀清热方低、中、高3个剂量组及秋水仙碱组大鼠血清IL-1β、TNF-α的水平降低(P<0.05)。与秋水仙碱组比较,祛瘀清热方低、中、高剂量组IL-1β、TNF-α水平增加(P<0.05)。与正常组比较,模型组及祛瘀清热方中、高剂量组滑膜组织NLRP6蛋白表达下调(P<0.05),模型组Caspase-1蛋白表达上调(P<0.05)。与模型组比较,祛瘀清热方高剂量组滑膜组织NLRP6蛋白表达上调(P<0.05),Caspase-1蛋白表达下调(P<0.05)。结论:祛瘀清热方可抑制GA炎性反应,降低血清中IL-1β、TNF-α含量,上调滑膜组织NLRP6蛋白和下调滑膜组织Caspase-1蛋白的表达。Objective:To observe the effect of Quyu Qingre Prescription on NLRP6/Caspase-1/IL-1βsignaling pathway in rats with gouty arthritis(GA) induced by sodium urate.Methods:The swelling degree of ankle joint was detected.The contents of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) in serum were determined by enzyme-linked immunosorbent assay(ELISA).The contents of NLRP6 and Caspase-1 in synovial tissue were detected by Western Blot.Results:Compared with the normal group,the swelling degree of ankle joint in other groups was significantly increased at different time points after modeling(P<0.05).Compared with the model group,the swelling degree of ankle joint in low-,mediumand high-dose groups of Quyu Qingre Prescription and colchicine group decreased at each time point after modeling(P<0.05).Compared with colchicine group,the swelling degree of ankle joint in low-,mediumand high-dose groups of Quyu Qingre Prescription increased at each time point after modeling(P<0.05).Serum levels of IL-1β and TNF-α in model group were higher than those in normal group(P<0.05).Compared with the model group,serum IL-1β and TNF-α levels were decreased in low-,medium-and high-dose groups of Quyu Qingre Prescription and colchicine group(P<0.05).Compared with colchicine group,the concentration levels of IL-1β and TNF-α in low-,medium-and high-dose groups of Quyu Qingre Prescription increased(P<0.05).Compared with the normal group,NLRP6 protein expression in synovial tissue of model group and medium-and high-dose groups of Quyu Qingre Prescription was downregulated(P<0.05),and Caspase-1 protein expression of model group was up-regulated(P<0.05).Compared with the model group,NLRP6 protein expression was up-regulated and Caspase-1 protein expression was down-regulated in synovial tissue of Quyu Qingre Prescription high-dose group(P<0.05).Conclusion:Quyu Qingre Prescription can inhibit the inflammatory response of GA,reduce the content of IL-1β and TNF-α in serum,up-regulate the expression of NLRP6 and down-regulate th

关 键 词：痛风性关节炎 祛瘀清热方 尿酸钠 炎性反应 

分 类 号：R684.3[医药卫生—骨科学]