不同支气管镜介导标本的结核分枝杆菌基因检测对痰涂片阴性肺结核患者的诊断价值  

Value of TB-DNA Test of Bronchoscopic Specimens in the Diagnosis of Sputum-negative Pulmonary Tuberculosis

作　　者：蔡向敬 吴丽娟 冯泮泮 Cai Xiangjing;Wu Lijuan;Feng Panpan(Department of Tuberculosis,Liaocheng Infectious Diseases Hospital,Liaocheng 252000,Shandong Province,China)

机构地区：[1]聊城市传染病医院结核科,山东聊城252000

出　　处：《中外医药研究》2023年第16期150-152,共3页JOURNAL OF CHINESE AND FOREIGN MEDICINE AND PHARMACY RESEARCH

摘　　要：目的:分析不同支气管镜介导标本结核分枝杆菌基因(TB-DNA)检测对痰涂片阴性肺结核患者的诊断价值。方法:选取2022年1—12月聊城市传染病医院痰涂片阴性肺结核患者178例为研究对象,采用奇偶数字法分为对照组和研究组,各89例。对照组采用肺泡灌洗液进行标本提取,研究组采用毛刷刷检进行痰液标本收集,两组均以荧光定量聚合酶链反应检测标本中的TB-DNA,比较两组肺结核诊断、耐药性检测效能以及不良反应。结果:研究组肺结核诊断灵敏度、特异度以及准确率均高于对照组,差异有统计学意义(P<0.05);研究组耐药性检测灵敏度、特异度以及准确率均高于对照组,差异有统计学意义(P<0.05);两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论:在痰涂片阴性肺结核患者的疾病诊断中,以毛刷刷检介导标本的肺结核诊断、耐药性检测效能优于肺泡灌洗液介导标本,两种标本介导方式不良反应均比较少,安全性相当。Objetive:To analyze the value of Mycobacterium tuberculosis(TB)-DNA test in bronchoscopic specimens in the diagnosis of sputum-negative pulmonary TB.Methods:178 patients with sputum-smear-negative pulmonary TB in Liaocheng City Infectious Diseases Hospital from January to December 2022 were selected for the study,and were divided into the control group and the study group according to the odd-even-numbered method,with 89 cases in each group.Bronchoalveolar lavage(BAL)for specimen extraction war carried out in the control group,while brushing for sputum specimen collection in the research group.Both groups detected TB-DNA in the specimens with fluorescent quantitation-PCR,and then TB diagnosis results,drug resistance,diagnostic efficacy,and adverse reactions between the two groups were compared.Results:The sensitivity,specificity and accuracy of TB diagnosis in the study group were higher than those in the control group,and the difference was statistically significant(P<0.05);the sensitivity,specificity and accuracy of drug resistance detection in the study group were higher than those in the control group,and the difference was statistically significant(P<0.05);and there was no statistically significant difference in the incidence of adverse reactions between the two groups(P>0.05).Conclusion:In the diagnosis of sputum-negative pulmonary TB patients,the efficacy of tuberculosis diagnosis and drug resistance detection by brushing-tested specimens is better than that of BAL-tested specimens,and the adverse reactions of the two types of specimens are fewer,and the safety is comparable.

关 键 词：肺结核 毛刷刷检 肺泡灌洗液 结核分枝杆菌基因 痰涂片 

分 类 号：R52[医药卫生—内科学]