刚地弓形虫RNA结合蛋白DDX39生物信息学分析  

Bioinformatics analysis of the RNA binding protein DDX39 of Toxoplasma gondii

作　　者：杨振科 王靖惠 齐一鸣 田晓薇 梅雪芳 张振超 王帅[1,2] YANG Zhenke;WANG Jinghui;QI Yiming;TIAN Xiaowei;MEI Xuefang;ZHANG Zhenchao;WANG Shuai(Department of Pathogenic Biology,School of Basic Medical Sciences,Xinxiang Medical University,Xinxiang,Henan 453003,China;Xinxiang Key Laboratory of Pathogenic Biology,Xinxiang,Henan 453003,China)

机构地区：[1]新乡医学院基础医学院病原生物学系,河南新乡453003 [2]河南省新乡市病原生物学重点实验室,河南新乡453003

出　　处：《中国血吸虫病防治杂志》2023年第4期358-365,373,共9页Chinese Journal of Schistosomiasis Control

基　　金：河南省科技攻关计划项目(232102311142);河南省高等学校重点科研项目(22A310004);新乡医学院人才支持计划(XYBSKYZZ202179);河南省高校大学生创新创业训练计划(202210472055)。

摘　　要：目的采用生物信息学技术对刚地弓形虫RNA结合蛋白DDX39(TgDDX39)进行分析,评估该蛋白的免疫原性,从而为研发弓形虫病疫苗提供参考依据。方法在ToxoDB数据库中检索TgDDX39氨基酸序列后,利用ProtParam、TMHMM 2.0、SignalP 5.0、NetPhos 3.1、COILS、SOPMA、Phyre2、ProtScale、ABCpred、SYFPEITHI、DNASTAR等生物信息学软件预测TgDDX39蛋白的理化性质、蛋白跨膜结构域、信号肽位点、翻译后修饰位点、卷曲螺旋、二级和三级结构、亲疏水性及抗原表位。结果Tg DDX39蛋白是一种不稳定亲水性蛋白,分子式C_(2173)H_(3458)N_(598)O_(661)S_(18),含有434个氨基酸,预测分子量为49.1 kDa,理论等电点为5.55。预测该蛋白存在信号肽的可能性极低,无跨膜区,并含有27个磷酸化位点;TgDDX39蛋白二级结构中β转角和无规则卷曲占39.63%,有1个部位有形成卷曲螺旋的倾向,且TgDDX39蛋白含有多个B、T细胞抗原表位。结论生物信息学预测显示,TgDDX39蛋白具有高免疫原性、含有多种抗原表位,具有作为弓形虫病疫苗候选蛋白分子的潜力。Objective To analyze the RNA binding protein of Toxoplasma gondii(TgDDX39) using bioinformatics technology,and to evaluate the immunogenicity of TgDDX39,so as to provide insights into development of toxoplasmosis vaccines.Methods The amino acid sequences of TgDDX39 were retrieved from the ToxoDB database,and the physicochemical properties,transmembrane structure domain,signal peptide sites,post-translational modification sites,coils,secondary and tertiary structures,hydrophobicity,and antigenic epitopes of the TgDDX39 protein were predicted using online bioinformatics tools,incluiding ProtParam,TMHMM 2.0,SignalP 5.0,NetPhos 3.1,COILS,SOPMA,Phyre2,ProtScale,ABCpred,SYFPEITHI and DNASTAR.Results TgDDX39 protein was predicted to be an unstable hydrophilic protein with the molecular formula of C_(2173)H_(3458)N_(598)O_(661)S_(18),which contained 434 amino acids and had an estimated molecular weight of 49.1 kDa and a theoretical isoelectric point of 5.55.The protein was predicted to have an extremely low possibility of signal peptides,without transmembrane regions,and contain 27 phosphorylation sites.The β turn and random coils accounted for 39.63% of the secondary structure of the TgDDX39 protein,and a coiled helix tended to produce in one site.In addition,the TgDDX39 protein contained multiple B and T cell antigenic epitopes.Conclusions Bioinformatics analyses predict that TgDDX39 protein has high immunogenicity and contains multiple antigenic epitopes.TgDDX39 protein is a potential candidate antigen for vaccine development.

关 键 词：刚地弓形虫 RNA结合蛋白 DDX39蛋白 生物信息学分析 免疫原性 

分 类 号：R382.5[医药卫生—医学寄生虫学]