肉苁蓉总苷对间歇低氧大鼠氧化应激及认知功能的影响  

Effect of total glucosides of Cistanche deserticola on oxidative stress and cognitive function in rats with intermittent hypoxia

作　　者：张盼盼 韩晓庆 王袁 黄超 王红阳 ZHANG Panpan;HAN Xiaoqing;WANG Yuan;HUANG Chao;WANG Hongyang(Department of Respiratory Medicine,North China University of Science and Technology Affiliated Hospital,Tangshan,Hebei 063000,P.R.China)

机构地区：[1]华北理工大学附属医院呼吸科,河北唐山063000

出　　处：《中国呼吸与危重监护杂志》2023年第4期269-273,共5页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：唐山市人才资助项目(A202110033)。

摘　　要：目的探讨肉苁蓉总苷对间歇低氧大鼠海马区氧化应激及认知功能的影响。方法选取成年雄性Wistar大鼠72只,采用随机数字表法分为3组:空白对照组、5%间歇低氧组(简称低氧组)、肉苁蓉总苷干预组(简称总苷干预组)。采用自制低氧舱模拟5%间歇低氧大鼠模型。分别将低氧组和总苷干预组给与5%间歇低氧,总苷干预组在给与5%间歇低氧的同时给与肉苁蓉总苷治疗。分别在第2、4、6、8周应用Morris水迷宫检测各组大鼠学习记忆功能。同时采用超氧化物歧化酶(superoxide dismutase,SOD)检测试剂盒检测各组大鼠海马区SOD的活性,采用丙二醛(malondialdehyde,MDA)检测试剂盒检测各组大鼠海马区MDA的含量。结果低氧组及总苷干预组在2、4、6、8周大鼠逃避潜伏期时间均较空白对照组显著延长(P<0.05)。而低氧组及总苷干预组的跨越目标象限时间则在2、4、6、8周均逐渐缩短(P<0.05)。与低氧组比较,总苷干预组在2、4、6、8周大鼠逃避潜伏期时间均逐渐缩短(P<0.05),而跨越目标象限时间则在2、4、6、8周均逐渐延长(P<0.05)。低氧组及总苷干预组海马组织MDA表达水平在2、4、6、8周均显著高于空白对照组(P<0.05),SOD表达水平在2、4、6、8周均显著低于空白对照组(P<0.05)。与低氧组比较,总苷干预组海马区神经细胞MDA表达水平在2、4、6、8周均降低,而SOD表达水平在2、4、6、8周均增加,差异有统计学意义(P<0.05)。结论肉苁蓉总苷可通过抑制间歇低氧大鼠的氧化应激,进而改善间歇低氧大鼠学习记忆功能。Objective To explore the effect of total glucosides of Cistanche deserticola on oxidative stress and cognitive function in rats with intermittent hypoxia.Methods Adult male Wistar rats(n=72)were randomly divided into three groups:a blank control group,a 5%intermittent hypoxia group(IH group)and a total glucosides of Cistanche deserticola intervention group(TGs intervention group).The 5%intermittent hypoxia rat model was simulated by using the self-made cabin of intermittent hypoxia.The rats in the IH group and the TGs intervention group were given 5%intermittent hypoxia respectively,and the rats in the TGs intervention group were treated with total glucosides of Cistanche deserticola simultaneously.Learning and memory function was tested by Morris water maze in three groups at the 2nd,4th,6th and 8th week respectively.The expressions of superoxide dismutase(SOD)and malondialdehyde(MDA)in hippocampus were detected by test kit.Results Compared with the blank control group,the escape latency time of the rats in the IH group and the TGs intervention group was significantly prolonged at the 2nd,4th,6th and 8th week respectively(P<0.05).The time to cross the target quadrant in the IH group and the TGs intervention group was gradually shortened at the 2nd,4th,6th and 8th week respectively(P<0.05).Compared with the IH group,the escape latency gradually shortened at the 2nd,4th,6th and 8th week in the TGs intervention group(P<0.05),while the time to cross the target quadrant was gradually prolonged at the 2nd,4th,6th and 8th week(P<0.05).The expressions of MDA in hippocampal tissue in the IH group and the TGs intervention group increased at the 2nd,4th,6th and 8th week(P<0.05),which were significantly higher than those in the blank control group;and the expressions of SOD at the 2nd,4th,6th,and 8th week were all lower than those in the blank control group(P<0.05).Compared with the IH group,the expression of MDA protein in hippocampal tissue in the TGs intervention group decreased at the 2nd,4th,6th and 8th week,while the

关 键 词：间歇低氧 肉苁蓉总苷 氧化应激 认知功能 

分 类 号：R285.5[医药卫生—中药学]