The nitrate-responsive transcription factor Md NLP7 regulates callus formation by modulating auxin response  

作　　者：LI Tong FENG Zi-quan ZHANG Ting-ting YOU Chun-xiang ZHOU Chao WANG Xiao-fei 

机构地区：[1]Apple Technology Innovation Center of Shandong Province/Shandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient Production/National Key Laboratory of Wheat Improvement/College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an 271018,P.R.China [2]National Key Laboratory of Wheat Improvement/College of Life Sciences,Shandong Agricultural University,Tai’an 271018,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第10期3022-3033,共12页农业科学学报（英文版）

基　　金：the National Natural Science Foundation of China(31972378);the Shandong Province Key R&D Program;China(2021CXGC010802);the China Agriculture Research System of MOF and MARA(CARS-27)。

摘　　要：Under appropriate culture conditions,plant cells can regenerate new organs or even whole plants.De novo organ regeneration is an excellent biological system,which usually requires additional growth regulators,including auxin and cytokinin.Nitrate is an essential nutrient element for plant vegetative and reproductive development.It has been reported that nitrate is involved in auxin biosynthesis and transport throughout the growth and development of plants.In this study,we demonstrated that the ectopic expression of the MdNLP7 transcription factor in Arabidopsis could regulate the regeneration of root explants.MdNLP7 mainly participated in the regulation of callus formation,starting with pericycle cell division,and mainly affected auxin distribution and accumulation in the regulation process.Moreover,MdNLP7 upregulated the expression of genes related to auxin biosynthesis and transport in the callus formation stage.The results demonstrated that MdNLP7 may play a role in the nitrate-modulated regeneration of root explants.Moreover,the results revealed that nitrate–auxin crosstalk is required for de novo callus initiation and clarified the mechanisms of organogenesis.

关 键 词：MdNLP7 callus initiation AUXIN NITRATE pericycle shoot regeneration 

分 类 号：Q943.2[生物学—植物学]