简易方法合成具有类过氧化物酶活性的聚乙烯亚胺包覆的四氧化三铁用于高灵敏检测干扰素α-2b  

Facile synthesis of Fe_(3)O_(4)@polyethylenimine with peroxidase-like activity for highly sensitive detection of interferon α-2b

作　　者：孙旭 常军亮 董青 王浩 张雪梅 李丹 高海成 汪尔康 汪劲 Xu Sun;Junliang Chang;Qing Dong;Hao Wang;Xuemei Zhang;Dan Li;Haicheng Gao;Erkang Wang;Jin Wang(College of Pharmacy,Jilin University,Changchun 130012,China;State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,CAS,Changchun 130022,China;Changchun Institute of Biological Products,Changchun 130015,China;Department of Chemistry and Physics,State University of New York at Stony Brook,Stony Brook,NY 11794-3400,USA)

机构地区：[1]College of Pharmacy,Jilin University,Changchun 130012,China [2]State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,CAS,Changchun 130022,China [3]Changchun Institute of Biological Products,Changchun 130015,China [4]Department of Chemistry and Physics,State University of New York at Stony Brook,Stony Brook,NY 11794-3400,USA

出　　处：《Science China Materials》2023年第10期4121-4130,共10页中国科学（材料科学（英文版）

基　　金：financially supported by the National Key Research and Development Program of China(2019YFA0709202);the Natural Science Foundation of Jilin Province(20220101055JC);the International Cooperation Project of Jilin Scientific and Technological Development Program(20190701059GH);the Department of Science and Technology of Jilin Province(20220508098RC)。

摘　　要：在病毒感染和癌症治疗中,干扰素α-2b(IFN-α2b)的灵敏检测至关重要,因此需要开发经济、稳定的灵敏检测IFN-α2b的方法.传统的酶联免疫吸附测定(ELISA)中使用的天然酶存在制备成本高和稳定性差等问题.为了提高其灵敏度并降低成本,我们合成了聚乙烯亚胺(PEI)修饰的四氧化三铁磁性纳米粒子(Fe_(3)O_(4)@PEI MNPs).在基于ELISA的IFN-α2b检测中,这些磁性纳米粒子作为辣根过氧化物酶的替代品,提供了比色谱和传统ELISA技术更高的灵敏度,并且能够实现IFN-α2b的可视化检测.该免疫分析方法的线性范围为0.075-25 ng mL^(-1),检测限为0.055 ng mL^(-1).基于Fe_(3)O_(4)@PEI MNPs优异的过氧化物酶活性,该方法在用于检测IFN-α2b和其他蛋白质生物标志物监测方面具有临床应用潜力.The sensitive detection of interferon alpha-2b(IFN-α2b)is crucial in treating viral infections and cancers.However,the natural enzymes used in the traditional enzymelinked immunosorbent assays(ELISA)face the obstacles of high cost and low stability.Therefore,there is an urgent need to develop more cost-effective and stable alternatives to detect IFN-α2b.To address this issue,we synthesized polyethyleneimine(PEI)-modified magnetic nanoparticles(Fe_(3)O_(4)@PEI MNPs)that are highly stable,affordable and simple to prepare.These Fe_(3)O_(4)@PEI MNPs serve as a substitute for horseradish peroxidase in the ELISA-based detection of IFN-α2b,providing better sensitivity than chromatographic and traditional ELISA techniques.The peroxidase-like activity of Fe_(3)O_(4)@PEI MNPs can develop color,enabling the visualization of IFN-α2b.The proposed immunoassay has a linear increase with IFN-α2b concentrations ranging from 0.075 to 25 ng mL^(−1),with a low limit of detection of 0.055 ng mL^(−1).Based on the outstanding peroxidase-like activity of Fe_(3)O_(4)@PEI MNPs,this method has the potential to be used for the clinical detection of IFN-α2b and other protein biomarkers in disease diagnosis and treatments.

关 键 词：one-step synthesis Fe_(3)O_(4) POLYETHYLENEIMINE magnetic nanoparticles ELISA interferonα-2b 

分 类 号：O657[理学—分析化学]