漆酶酶促丝素接枝壳聚糖及其膜材料的制备  

Preparation of chitosan grafted silk fibroin with laccase and its membrane materials

作　　者：陈静 彭泽冶 黄继伟[1] 林海涛[1] 宁晚娥[1] CHEN Jing;PENG Zeye;HUANG Jiwei;LIN Haitao;NING Wan'e(College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,Guangxi,China;Hunan Bide Biochemical Technology Co.,Ltd.,Yueyang 414306,Hunan,China)

机构地区：[1]广西科技大学生物与化学工程学院,广西柳州545006 [2]湖南比德生化科技股份有限公司,湖南岳阳414306

出　　处：《上海纺织科技》2023年第10期60-65,81,共7页Shanghai Textile Science & Technology

基　　金：国家自然科学基金项目(51763001、51963002);广西自然科学基金项目(2018GXNSFAA281280);柳州市科技计划项目(2019DH106 02)。

摘　　要：为改善丝素膜的性能,采用漆酶将壳聚糖接枝到丝素上,制备成具有抗菌性能的复合丝素膜,探讨了壳聚糖质量分数和漆酶对丝素膜结构及其性能的影响。结果显示:酶促丝素接枝壳聚糖的反应溶液中大分子量的蛋白含量增多,证明丝素溶液在发生自交联时还可以接枝壳聚糖;当壳聚糖质量分数为4 g/L时,在酶促丝素接枝壳聚糖后,丝素膜材料在水中的溶失率最小,而溶胀率随着壳聚糖质量分数的增加而增加,且丝素膜材料的表面形貌没有发生明显变化;漆酶催化丝素接枝壳聚糖对丝素蛋白的结晶结构有一定影响;经漆酶催化丝素接枝壳聚糖后,丝素膜的力学性能提升,丝素膜具有了一定的抗菌性能。In order to improve the performance of silk fibroin membrane,chitosan is grafted onto silk fibroin using laccase to prepare composite silk fibroin chitosan was grafted onto silk fibroin using laccase to prepare composite silk fibroin films with antibacterial properties with antibacterial properties.The effects of chitosan dosage and laccase on the structure and properties of silk fibroin membrane are investigated.The results show that the high molecular weight protein content in the reaction solution of enzymatic silk fibroin grafting chitosan increases,indicating that silk fibroin solution could graft chitosan during self-crosslinking.When the dosage of chitosan is 4 g/L,after enzymatic fibroin grafting of silk fibroin onto chitosan,the solubility of silk fibroin membrane material in water is the smallest,while the swelling rate increases with the increase of chitosan mass fraction,and the surface morphology of silk fibroin membrane material does not change significantly.Laccase catalyzed grafting of silk fibroin onto chitosan has a certain effect on the crystal structure of silk fibroin.In addition,the mechanical properties of silk fibroin membrane can be improved,and the silk fibroin membrane exhibits certain antibacterial properties.

关 键 词：漆酶 丝素蛋白 壳聚糖 接枝 膜材料 交联 

分 类 号：TS102.333[轻工技术与工程—纺织工程]