核酸分子开关稳定性增强策略  

Enhanced strategy for switching stability of nucleic acid molecules

作　　者：薛倩 王焕翔 邹振 Xue Qian;Wang Huan-xiang;Zhou Zhen(Hunan Provincial Key Laboratory of Cytochemistry,School of Chemistry and Chemical Engineering,Changsha University of Science and Technology,Changsha,,410114 China)

机构地区：[1]长沙理工大学化学化工学院细胞化学湖南省重点实验室,长沙410114

出　　处：《化学传感器》2023年第2期34-43,共10页Chemical Sensors

基　　金：国家自然科学基金(21705010,21735001,21605008);湖南省教育厅优秀青年项目(20B032);湖南省研究生创新项目(CX20200890)。

摘　　要：核酸分子开关由核酸分子自组装形成,包括核酸分子识别单元和荧光信号报告单元。核酸分子开关具有设计简便、易合成、易修饰、特异性高等优点被广泛用于化学生物传感领域。然而,在实际检测体系中,核酸探针的稳定性是阻碍其应用的瓶颈问题。在生物样品中,核酸分子开关易被各种核酸酶降解,产生假阳性信号,影响检测的准确度。该文主要介绍为核酸分子开关的生物稳定性改善方法,重点介绍碱基修饰改性、核酸纳米框架结构、纳米材料负载、封闭核酸酶切割位点、左旋核酸等保护策略。Nucleic acid molecular switch is formed by self-assembly of nucleic acid molecules,including nucleic acid molecular recognition unit and fluorescent signal reporting unit.Nucleic acid molecular switches are widely used in the field of chemical and biological sensing because of their simple design,easy synthesis,easy modification and high specificity.However,in the practical detection system,the stability of nucleic acid probe is the bottleneck problem that hinders its application.In biological samples,nucleic acid molecular switches are easy to be degraded by various nucleases,resulting in false positive signals,which affect the accuracy of detection.This paper mainly introduces the methods to improve the biological stability of nucleic acid molecular switches,focusing on the protection strategies such as base modification,nucleic acid nano framework structure,nano material loading,closing nuclease cleavage sites,left-handed nucleic acid,etc.

关 键 词：核酸分子开关 生物传感 抗酶解性能 生物稳定性 

分 类 号：O657.3[理学—分析化学] TP212[理学—化学]