磷限制对球形棕囊藻生长及TEP形成的影响  

Effect of Phosphorus Limitation on the Growth and TEP Formation of Phaeocystis Globose

作　　者：蓝彩碧 李杰[1,2] 赖俊翔 许铭本[1,2,3] LAN Caibi;LI Jie;LAI Junxiang;XU Mingben(Guangxi Academy of Sciences,Nanning 530007,China;Guangxi Key Laboratory of Marine Environmental Science(Guangxi Academy of Marine Sciences),Nanning 530007,China;Beibu Gulf Marine Industry Research Institute,Fangchenggang 538001,China)

机构地区：[1]广西科学院,广西南宁530007 [2]广西近海海洋环境科学重点实验室(广西海洋科学院),广西南宁530007 [3]北部湾海洋产业研究院,广西防城港538001

出　　处：《海洋技术学报》2023年第5期94-101,共8页Journal of Ocean Technology

基　　金：中央引导地方科技发展资金资助项目(桂科ZY21195027);国家自然科学基金资助项目(41506146);广西科技重大专项项目(桂科AA17202020)。

摘　　要：广西北部湾近岸海域虽常年为磷限制状态,但球形棕囊藻(Phaeocystis Globosa)赤潮灾害频繁发生。球形棕囊藻暴发时主要以囊体形式存在,赤潮衰退后,囊体破裂产生大量的透明胞外聚合颗粒物(Transparent Exopolymer Particles,TEP),在海洋动力作用下形成高碳、高黏性泡沫,影响渔业和核电取水安全。本文通过设置氮磷比为32∶1和64∶1的半连续培养实验,探讨磷限制对球形棕囊藻的生长,以及透明胞外聚合颗粒物产量的影响。结果表明:在氮磷比为32∶1和64∶1的磷限制条件下,球形棕囊藻的细胞密度、叶绿素a浓度、囊体密度及囊体表面细胞数,在培养的后半期都受到了抑制。与控制组相比,两个磷限制的处理组在囊体直径上并没有表现出显著的差异(显著性水平p>0.05)。在氮磷比为32∶1的条件下,球形棕囊藻的TEP生产量与控制组相比没有显著差异(p>0.05),在氮磷比为64∶1的条件下,TEP的生产量显著降低(p<0.05),磷限制程度的加剧对球形棕囊藻细胞分泌TEP产生影响。研究结果为进一步研究环境胁迫下球形棕囊藻TEP形成机制提供了依据。Although the coastal waters of Beibu Gulf in Guangxi are in a phosphorus restricted state all the year round,the bloom of Phaeocystis Globosa occurs frequently.During the outbreak,Phaeocystis globosa mainly exists in the form of colony.After the bloom recession,the colony burst and produce a large number of Transparent Exopolymer Particles(TEP),which form a high-carbon and highviscosity foam under the action of ocean dynamics,affecting the safety of fisheries and nuclear power water intake.This article explores the effects of phosphorus limitation on the growth of Phaeocystis Globosa and the production of transparent extracellular polymeric particles through semi-continuous culture experiments with nitrogen phosphorus ratios of 32:1 and 64:1.Exploring the effects of phosphorus limitation on the growth and production of transparent extracellular polymeric particulate matter in the Phaeoystis globosa.The results showed that cell density、chlorophyll a concentration、colony density and colony surface cell number of Phaeocystis Globosa were all inhibited in the second half of culture under the phosphorus restriction of nitrogen phosphorus ratios of 32:1 and 64:1.However,the two phosphorus-limited experimental groups did not show significant differences in colony diameter compared to the control group(Significance level p>0.05).Under the condition of nitrogen phosphorus ratios of 32:1,TEP production of Phaeocystis Globosa was not significantly different from that of control group(p>0.05),and under the condition of nitrogen phosphorus ratios of 64:1,TEP production was significantly decreased(p<0.05).The increased degree of phosphorus restriction had an effect on TEP secretion of Phaeocystis Globosa cells.The results provided a basis for further study of TEP formation mechanism of Phaeocystis Globosa under environmental stress.

关 键 词：球形棕囊藻 赤潮 生长 透明胞外聚合颗粒物 磷限制 

分 类 号：P76[天文地球—海洋科学]