短链氯化石蜡长期低剂量暴露对小鼠肝脏的影响  

Effects of Long-term Low-dose Exposure to Short-chain Chlorinated Paraffins on mouse liver

作　　者：黎威巍 马萍[1] 杨旭[1] 晏彪[1] LI Wei-wei;MA Ping;YAN Biao(Research Center of Basic Medical Sciences,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院基础医学研究中心,湖北咸宁437100

出　　处：《湖北科技学院学报（医学版）》2023年第5期390-394,共5页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北科技学院校级项目(BK202319)。

摘　　要：目的本研究旨在探究0.7μg/kg短链氯化石蜡(SCCPs)暴露对昆明小鼠肝脏功能及其代谢的影响。方法雄性昆明小鼠随机分为对照组、SCCPs暴露组,每组各8只,48d后收集肝脏,检测肝功能生化指标并进行组织病理学分析、Hoechst 33258荧光观察和能量代谢相关检测。结果与对照组比较,SCCPs暴露组肝功能生化指标显著升高(P<0.05),肝细胞肿大,可见空泡化病理结构,肝细胞线粒体形态和结构损伤,线粒体氧化代谢紊乱。结论长期低剂量SCCPs暴露可引起小鼠肝功能及代谢异常,作用机制或与其致肝细胞线粒体功能障碍有关。Objective The aim of this study was to investigate the effects of 0.7μg/kg short-chain chlorinated paraffins(SCCPs)exposure on liver function and its metabolism in Kunming mice.Methods Male Kunming mice were randomly divided into control and SCCPs-exposed groups(n=8 mice),and livers were collected after 48 days for the biochemical indices of liver function and histopathological analysis,Hoechst 33258 fluorescence observation and energy metabolism-related assays.Results Compared with the control group,the SCCPs-exposed group showed significantly higher biochemical parameters of liver function(P<0.05),hepatocyte enlargement,visible vacuolated pathological structures,damaged hepatocyte mitochondrial morphology and structure,and disrupted mitochondrial oxidative metabolism.Conclusion Long-term low-dose SCCPs exposure could still cause abnormal liver function and metabolism in mice,and the mechanism may be related to mitochondrial dysfunction in hepatocytes caused by SCCPs exposure.

关 键 词：短链氯化石蜡 毒性效应 肝功能障碍 肝代谢异常 

分 类 号：R96[医药卫生—药理学]