碘对甲状腺细胞组蛋白甲基化修饰的影响  

Changes of histone methylation modification in thyroid cells induced by iodine

作　　者：路晰媗 雷红 徐礼 梁海艳 周小莉 沙立萍 LU Xixuan;LEI Hong;XU Li;LIANG Haiyan;ZHOU Xiaoli;SHA Liping(Department of Endocrinology,Cardiovascular and Cerebrovascular Disease Hospital,General Hospital of Ningxia Medical University,Yinchuan 750002,China)

机构地区：[1]宁夏医科大学总医院心脑血管病医院内分泌科,宁夏银川750002

出　　处：《宁夏医学杂志》2023年第10期871-874,F0002,共5页Ningxia Medical Journal

基　　金：宁夏自然科学基金项目(2020AAC03424)。

摘　　要：目的 从表观遗传的角度探讨碘对甲状腺疾病的影响,分析碘化钠作用于甲状腺细胞后组蛋白甲基化及相关修饰酶的变化。方法 用不同浓度碘化钠(1μM,10μM,100μM,1 mM,10 mM)刺激甲状腺细胞系N-thy-ori-3-1后,通过蛋白质印迹法和荧光定量PCR检测了蛋白表达和转录水平。结果 不同浓度碘化钠作用于甲状腺细胞后引起细胞组蛋白H3赖氨酸4的三甲基化(H3K4me3)整体蛋白水平的升高,同时组蛋白H3赖氨酸27的三甲基化(H3K27me3)整体蛋白水平下降,二者变化呈相反趋势,H3K4me3总蛋白随着碘化钠浓度升高逐渐增高,并随着时间动态改变,同时碘化钠能诱导升高相关甲基化转移酶MLL1和去甲基化转移酶JMJD3的转录表达和蛋白水平。结论 碘能诱导甲状腺细胞整体H3K4me3蛋白的表达增加和H3K27me3蛋白的表达减少,伴随MLL1和JMJD3的表达增加,为进一步探讨高碘与甲状腺自身免疫的机制研究提供了表观学的研究基础。Objective To explore the effects of iodine on thyroid diseases from the perspective of epigenetic inheritance,and analyze the changes of histone methylation and related modifying enzymes after the action of sodium iodide on thyroid cells.Methods After stimulating thyroid cell line N-thy-ori3-1 with different concentrations of sodium iodide(1μM,10μM,100μM,1 mM,10 mM),the expression levels of protein and mRNA were detected by western blotting and RT-PCR.Results The overall protein level of trimethylated histone H3 lysine 4(H3K4me3)in thyroid cells increased after the different concentrations of sodium iodide,while the total protein level of trimethylated histone H3 lysine 27(H3K27me3)decreased.The two changes showed a transient opposite trend.The total protein of H3K4me3 increased gradually with the increase of sodium iodide concentration,and dynamically changed with time.At the same time,sodium iodide could increase the expression levels of mRNA and protein of related methyltransferases MLL1 and demethyltransferase JMJD3.Conclusion Iodine can increase the level of H3K4me3 and decrease the level of H3K27me3 in thyroid cells,accompanied by an increase in the expression of MLL1 and JMJD3,which provides an epigenetic basis for further exploring the mechanism of iodine on thyroid autoimmunity.

关 键 词：碘 组蛋白甲基化 表观遗传 甲状腺 

分 类 号：R581.4[医药卫生—内分泌]