我国锈色棕榈象遗传多样性及遗传结构分析  

Genetic diversity and structure analysis of Rhynchophorus ferrugineus(Olivier)in China

作　　者：肖雨倩 黄梦伊 阎伟[2] 石娟[1] XIAO Yuqian;HUANG Mengyi;YAN Wei;SHI Juan(The College of Forestry of Beijing Forestry University,Beijing 100089,China;Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wenchang,Hainan 571300,China)

机构地区：[1]北京林业大学林学院,北京100089 [2]中国热带农业科学院椰子研究所,海南文昌571300

出　　处：《生物安全学报》2023年第4期323-332,共10页Journal of biosafety

基　　金：国家重点研发计划项目(2021YFC2600400);国家自然科学基金项目(32171794);江西省林业厅林业科技创新专项(201912)。

摘　　要：【目的】外来入侵种锈色棕榈象是全国林业检疫性有害生物。本研究旨在探讨锈色棕榈象在我国的种群遗传结构和分化规律,旨在为今后锈色棕榈象传播蔓延控制提供一定依据。【方法】基于微卫星技术测定12个锈色棕榈象中国地理种群在9个微卫星位点上的序列,使用PopGen32、BOTTLENECK、STRUCTURE等软件对锈色棕榈象种群间的遗传多样性和遗传结构进行分析。【结果】12个地理种群中,有9个种群具有较高的遗传多样性(Nei′s平均值>0.5);瓶颈效应的检测结果显示,除云南蒙自的锈色棕榈象种群在近期遭遇了瓶颈效应以外,其他种群近期均未经历瓶颈效应;广西和江西的锈色棕榈象种群在大多数位点符合哈迪温伯格平衡,其他种群在9个微卫星位点中基本都偏离该平衡;12个地理种群在9个SSR位点的F_(st)值大部分都小于0.18,平均值为0.1769,基因流N_(em)为0.4332~2.1130;Structure和主坐标分析法(PcoA)将12个地理种群划分为3支。【结论】锈色棕榈象种群间主要的遗传变异发生在种群内部,种群间遗传变异的变化并不明显,大部分种群间存在频繁的基因交流;新入侵地江西新余和四川崇州的地理种群可能由福建传入。中国锈色棕榈象种群可能存在多个入侵源和入侵路径。【Aim】The invasive alien species Rhynchophorus ferrugineus Oliver is a national forestry quarantine pest.This study aimed to investigate the population genetic structure and differentiation rules of R.ferrugineus in China and to provide a basis for control of the spread in the future.【Method】The sequences of 12 Chinese geographic populations of R.ferrugineus at nine microsatellite loci were determined based on microsatellite technology,and the genetic diversity and genetic structure among populations of R.ferrugineus were analyzed using PopGen32,BOTTLENECK,and STRUCTURE software.【Result】Among the 12 geographic populations,nine had high genetic diversity(Nei′s mean>0.5).The results of the bottleneck effect test showed that,except for the population of Mengzi,which recently suffered from the bottleneck effect,other populations did not experience the bottleneck effect.The populations of Guangxi and Jiangxi conformed to the Hardy-Weinberg equilibrium at most loci,and other populations deviated from this equilibrium in nine microsatellite loci.The F_(st)of nine SSR loci in most of the 12 geographic populations was less than 0.18,the value of the mean was 0.1769,and N_(em)ranged from 0.4332 to 2.1130.The 12 populations were divided into three groups by the methods of STRUCTURE and principal coordinate analysis.【Conclusion】The main genetic variation among populations of R.ferrugineus occurred within the population,and the variation among populations was not obvious.Frequent gene exchange existed among most populations.R.ferrugineus from Xinyu,Jiangxi,and Chongzhou,Sichuan were probably introduced from Fujian.There might be multiple invasion sources and invasion paths in populations of R.ferrugineus in China.

关 键 词：锈色棕榈象 微卫星 遗传多样性 遗传结构 

分 类 号：S763.38[农业科学—森林保护学]