广西百色西番莲茎基腐病病原菌分离与鉴定  

Isolation and identification of the pathogens of Passiflora coerulea L.stem rot in Baise,Guangxi

作　　者：杨翠凤 滕峥 余爵运 林有林 YANG Cui-feng;TENG Zheng;YU Jue-yun;LIN You-lin(Agricultural and Food Engineering College,Baise University,Baise 533000,Guangxi,China;Baise Corn Research Institute,Baise 533604,Guangxi,China)

机构地区：[1]百色学院农业与食品工程学院,广西百色533000 [2]百色市玉米研究所,广西百色533604

出　　处：《湖北农业科学》2023年第10期69-73,共5页Hubei Agricultural Sciences

基　　金：百色市科技支撑计划项目(百科20203203);广西科技基地和人才专项(2020AC20047);广西自然科学基金项目(2021JJB130444,2022JJB130088);广西一流学科建设项目(农业资源与环境,桂教科研[2022]1号)。

摘　　要：采用组织分离法、科赫法则及rDNA-ITS测序鉴定等方法,对广西百色市西番莲(Passiflora coerulea L.)茎基腐病致病菌进行分离、培养和鉴定。结果表明,分离鉴定获得6种不同形态和致病力的病原真菌,其中4株分别鉴定为尖孢镰刀菌(Fusarium oxysporum)、烟草疫霉菌(Phytophthora nicotianae)、焦腐病菌(Lasiodiplodia theobromae)和串珠镰刀菌(Fusarium moniliforme),2株为镰刀菌属(Fusarium),但尚未鉴定到种。The pathogen of Passiflora coerulea L.stem rot in Baise City,Guangxi were isolated,cultured and identified by means of tissue separation,Koch’s rule and rDNA-ITS sequencing.The results showed that 6 kinds of pathogenic fungi with different morphology and pathogenicity were isolated and identified.Four strains were identified as Fusarium oxysporum,Phytophthora nicotianae,Lasiodiplodia theobromae and Fusarium moniliforme,2 strains were identified as Fusarium,but their the species had not been identified.

关 键 词：西番莲(Passiflora coerulea L.) 茎基腐病 镰刀菌属(Fusarium) 分离 鉴定 

分 类 号：S436.679[农业科学—农业昆虫与害虫防治]