山桐子性别分子标记的通用性验证  

Universality validation of molecular markers for sex identification in Idesia polycarpa Maxim.

作　　者：严莉 仝铸[1] 何秀娟[1] 肖翠 王泽琼 袁龙义[2] 孙中海[1] 邱文明[1] YAN Li;TONG Zhu;HE Xiu-juan;XIAO Cui;WANG Ze-qiong;YUAN Long-yi;SUN Zhong-hai;QIU Wen-ming(Institute of Fruit and Tea,Hubei Academy of Agricultural Sciences/Fruit and Tea Subcenter of Hubei Innovation Center of Agricultural Science and Technology,Wuhan 430064,China;College of Horticulture and Gardening,Yangtze University,Jingzhou 434025,Hubei,China)

机构地区：[1]湖北省农业科学院果树茶叶研究所/湖北省农业科技创新中心果树茶叶研究分中心,武汉430064 [2]长江大学园艺园林学院,湖北荆州434025

出　　处：《湖北农业科学》2023年第10期109-114,共6页Hubei Agricultural Sciences

基　　金：湖北省农业科技创新中心资助项目(2021-620-000-001-23)。

摘　　要：采用已知性别的120份山桐子(Idesia polycarpa Maxim.)样品,检验了2个已报道UBC841和STZ分子标记在山桐子性别鉴定中的通用性。同时,分析了大量简单重复序列间区扩增多态性(ISSR)和相关序列扩增多态性(SRAP)标记在山桐子雌雄株中的多态性。结果表明,UBC841标记在24份山桐子样品中可检测到多态性条带,但未检测到雌雄性别特异性条带;STZ标记可在部分样本中扩增出目的条带,但未表现出雌雄株性别特异性;说明这2组标记均未表现出良好的通用性,雌雄性别鉴别准确率较低。此外,筛选了26条ISSR引物和780对SRAP引物组,也未能获得雌雄性别特异标记,说明采用通用型分子标记ISSR和SRAP在山桐子中较难筛选到稳定、可靠的性别特异的标记。Using 120 samples of Idesia polycarpa with known sex,the universality of the two reported molecular markers UBC841 and STZ was tested for sex determination of Idesia polycarpa.Meanwhile,a large number of inter-simple sequence repeat(ISSR)and sequence related amplified polymorphisms(SRAP)markers were analyzed for their polymorphism in male and female plants of Idesia polycarpa.The results showed that UBC841 could detect polymorphic bands in 24 samples of Idesia polycarpa,but no gender-specific bands were detected.STZ could amplify the target band in some samples,but did not show the sex specificity of male and female bands.These two groups of markers did not show good universality,and the accuracy of gender discrimination was low.In addition,26 ISSR primers and 780 pairs of SRAP primers were screened,but no sex-specific markers were obtained,indicating that it was difficult to screen stable and reliable sex-specific markers in Idesia polycarpa by using universal molecular markers ISSR and SRAP.

关 键 词：山桐子(Idesia polycarpa Maxim.) 性别鉴定 简单重复序列间区扩增多态性(ISSR) 相关序列扩增多态性(SRAP) 通用性验证 

分 类 号：Q945.61[生物学—植物学]