草鱼摄食前后促食欲因子和抑制食欲因子的变化规律  

Pre-and postprandial changes of orexigenic factors and anorexigenic factors in grass carp(Ctenopharyngodon idella)

作　　者：郭薇 杨慧君[1] 莫爱杰 孙俊霄 翟昱翔 矣林园 袁勇超[1] GUO Wei;YANG Hui-jun;MO Ai-jie;SUN Jun-xiao;ZHAI Yu-xiang;YI Lin-yuan;YUAN Yong-chao(College of Fisheries,Huazhong Agricultural University/Key Lab of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding,Ministry of Agriculture and Rural Affairs,Wuhan 430070,China)

机构地区：[1]华中农业大学水产学院/农业动物遗传育种与繁殖教育部重点实验室/农业农村部淡水生物繁育重点实验室,武汉430070

出　　处：《湖北农业科学》2023年第10期143-149,共7页Hubei Agricultural Sciences

摘　　要：通过荧光定量PCR检测了草鱼(Ctenopharyngodon idella)摄食前后脑和肠道组织中胃饥饿素(Ghrelin)、神经肽Y(Neuropeptide Y,NPY)、可卡因-苯丙胺调节转录肽(Cocaine amphetamine regulated transcript,CART)、胆囊收缩素(Cholecystokinin,CCK)和瘦素(Leptin)的基因表达,探究食欲因子的变化规律。结果表明,摄食前2 h,草鱼脑组织中ghrelin mRNA表达水平达到最大峰值(P<0.05),随后显著下降,在投喂后的4 h内,其表达水平没有显著性变化(P>0.05)。脑中促食欲因子npy mRNA在摄食前2 h内的表达量显著高于摄食后2 h(P<0.05),而在肠道中表达量存在相反的变化趋势。脑和前肠组织中抑制食欲因子cck、cart和leptin mRNA在摄食前2 h表达丰度较低,而在摄食后1~2 h内,其含量显著上升(P<0.05)。摄食后草鱼血浆中GH含量表现出先上升后下降的趋势,并在摄食后1 h达到高峰。The transcriptional levels of ghrelin,npy,cart,cck and leptin in the brain and intestinal tissues of grass carp before and after feeding were detected by real-time fluorescence quantitative PCR to explore the variation laws of their mRNA transcripts.The results showed that transcription levels of ghrelin mRNA in the brain of grass carp reached the maximum peak 2 h before feeding(P<0.05),and then decreased significantly,but there were no significant changes in the expression levels within 4 h after feeding(P>0.05).The mRNA expression level of npy in the brain was significantly higher within 2 h before feeding than 2 h after feeding(P<0.05),while its mRNA expression presented an opposite trend in the foregut.Meanwhile,the expression levels of anorexigenic factors(cck,cart and leptin)in brain and foregut kept stable at a low level 2 h before feeding,while a significant increase was observed 1~2 h after feeding in the fed group(P<0.05).The GH content in plasma after feeding exhibited a trend of rising first and then decreasing,the peak value was delayed to 1 h after feeding.

关 键 词：草鱼(Ctenopharyngodon idella) 促食欲因子 抑制食欲因子 GH 

分 类 号：S917.4[农业科学—水产科学]