苦玄参种子的乙醇提取物的抗炎机制研究  

Mechanism Research on Alcohol Extract of Picria felterrae Lour.′s Seeds

作　　者：陈晖[1] 翁志华 李文玲 丘琴[2] CHEN Hui;WENG Zhi-hua;LI Wen-ling;QIU Qin(The people′s hospital of Liuzhou,Liuzhou 545006,China;The traditional medicine university of Guangxi,Nanning 530006,China)

机构地区：[1]柳州市人民医院,广西柳州545006 [2]广西中医药大学,广西南宁530006

出　　处：《海峡药学》2023年第9期10-14,共5页Strait Pharmaceutical Journal

基　　金：广西中医药管理局自筹基金课题,基金合同编号:GZZC2019209。

摘　　要：目的对苦玄参种子的乙醇提取物进行体外活性研究。方法在适宜条件下用RAW264.7细胞构建诱导炎症模型脂多糖(LPS),采用MTT法测定细胞活性,采用Griess法和ELISA法分别测定炎症因子TNF-α,IL-6和NO的释放量,采用Westernblot法测定p65、p-IκB、p38、JNK、ERK1/2蛋白的表达水平。结果苦玄参种子乙醇提取物能显著抑制LPS刺激RAW264.7细胞分泌的NO水平,且呈量效关系。抗炎活性表明,苦玄参种子乙醇提取物能显著下调炎症因子IL6、TNF-α的合成,并抑制了RAW264.7细胞中NF-κB信号通路中p-IκB-α和p65的磷酸化表达,以及MAPKs信号通路中p38、JNK、ERK1/2的磷酸化表达。结论苦玄参种子提取物具有一定的抗炎作用。OBJECTIVE To study the in vitro activity of Alcohol Extract of Picria felterrae Lour.′s Seeds.METHODS Establishing the inducing inflammation model induced by lipopolysaccharide on RAW264.7 cell under the suitable condition.Testing the activity of cell with MTT method,Testing the releasing amount of TNF-α,IL-6 and NO with Griess and ELISA method.Measuring the expressing protein level of p65 and p-IκB、p38,JNK,ERK1/2 by western blot.RESULTS The result suggested that the extract in alcohol of Picria felterrae Lour.′s seeds could restrain the lipopolysaccharide stimulating RAW264.7 cell the generation of NO,which was in effect-dose relationship.The activity of anti-inflammatory showed that the extract in alcohol of Picria felterrae Lour.′s seeds could lower the synthesis of IL-6,TNF-α.At the same time,it also could inhibit the phosphorylation expression of p-IκB-αand p65,as well as p38,JNK,ERK1/2 in NF-κB signal path and MAPKs signal path in RAW264.7 cell,respectively.CONCLUSION The result suggests that the extract of Picria felterrae Lour.′s seeds have the effect of anti-inflammatory to some extent.

关 键 词：苦玄参种子 RAW264.7细胞 炎症 

分 类 号：R965[医药卫生—药理学]