对甘蔗线条花叶病毒存在抗性差异的2个甘蔗品种中eIF4E基因序列分析  

Sequence analysis of eukaryotic translation initiation factor eIF4E from two varieties of sugarcane with differential resistance to sugarcane streak mosaic virus

作　　者：单红丽[1] 陈都 王晓燕[1] 张荣跃[1] 李婕 王长秘 李银煳 黄应昆 SHAN Hongli;CHEN Du;WANG Xiaoyan;ZHANG Rongyue;LI Jie;WANG Changmi;LI Yinhu;HUANG Yingkun(Sugarcane Research Institute,Yunnan Academy of Agricultural Science,Yunnan Key Laboratory of Sugarcane Genetic Improvement,Kaiyuan 661699,China)

机构地区：[1]云南省农业科学院甘蔗研究所云南省甘蔗遗传改良重点实验室,开远661699

出　　处：《植物病理学报》2023年第4期624-632,共9页Acta Phytopathologica Sinica

基　　金：云南省技术创新人才培养对象项目(202005AD160012);国家自然科学基金项目(31701490);财政部和农业农村部:国家现代农业产业技术体系(CARS-170303);云岭产业技术领军人才培养项目(2018LJRC56);云南省现代农业产业技术体系建设专项资金(YNGZTX-4-92)。

摘　　要：甘蔗线条花叶病毒(sugarcane streak mosaic virus,SCSMV)是近年引起甘蔗花叶病的主要病原,选育和利用抗病品种是防控甘蔗花叶病最经济有效的手段。不同品种对甘蔗花叶病具有不同抗性,明确抗性差异的来源对于选育抗病品种具有重要意义。本研究以SCSMV的高抗品种CP94-1100和高感品种ROC22为对象,对两个品种的致病病毒和真核生物翻译起始因子4E(eukaryotic translation initiation factor 4E,eIF4E)序列进行分析。在致病病毒方面,侵染CP94-1100和ROC22的SCSMV分离物cp基因和vpg基因核苷酸序列和蛋白编码情况基本相同,为同一分子变种。在寄主eIF4E方面,从CP94-1100和ROC22中均分离到eIF4E基因,均包含由663 bp构成的ORF,编码1个含220个氨基酸残基的多肽,氨基酸序列同源性仅为98.2%,说明抗性差异可能与eIF4E差异相关。生物信息学分析显示,CP94-1100和ROC22中eIF4E基因编码的蛋白均为稳定亲水性蛋白,属于IF4E家族蛋白,而CP94-1100中eIF4E基因编码的蛋白还包含1个CDC 33或PTZ00040的结构域。遗传分析表明eIF4E基因与禾本科其他作物高度相似,其中CP94-1100的eIF4E基因与割手密亲缘关系最近,说明它们也具有类似的功能。Sugarcane streak mosaic virus(SCSMV)is the main pathogen causing sugarcane mosaic disease in recent years.Breeding and using resistant varieties is the most economical and effective means to control sugarcane mosaic disease.Different varieties have different resistance to sugarcane mosaic disease.It is important to identify the source of resistance difference for breeding resistant varieties.In this study,the sequences of pathogenic viruses and eukaryotic translation initiation factor 4E(eIF4E)were analyzed in the highly resistant variety CP94-1100 and the highly susceptible variety ROC22.The results showed that SCSMV isolated from CP94-1100 and ROC22 were similar in the nucleotide sequences and amino acid sequences for cp gene and vpg gene,and were the same variant.The eIF4E gene was obtained from CP94-1100 and ROC22,which contained a 663 bp ORF encoding a polypeptide with 220 amino acid residues.The amino acid sequence homology of the eIF4Es from these two varieties was 98.2%,indicating a possible correlation between the eIF4E polymorphism and difference in resistance to SCSMV.Bioinformatics analysis showed that the IF4E family proteins encoded by the eIF4E genes in CP94-1100 and ROC22 were stable hydrophilic proteins.In addition,this IF4E family protein encoded by the eIF4E gene in CP94-1100 also contains a CDC33 or PTZ00040 domain.Genetic analysis showed that these eIF4E genes in CP94-1100 and ROC22 were highly similar to other eIF4E family genes from grami-neae,and the eIF4E gene of CP94-1100 was most closely related to that of Saccharum spontaneum,suggesting that they had similar functions.

关 键 词：甘蔗线条花叶病毒 抗性差异 外壳蛋白 病毒基因组连接蛋白 真核生物翻译起始因子4E 

分 类 号：S432.41[农业科学—植物病理学]