引起我国葡萄黑根病的Dactylonectria novozelandica的鉴定  

Identification of Dactylonectria novozelandica causing black foot disease of grapevine in China

作　　者：谭海芸 吴林娜 张玮[1] 陈鹏召 李兴红[1] 燕继晔[1] TAN Haiyun;WU Linna;ZHANG Wei;CHEN Pengzhao;LI Xinghong;YAN Jiye(Beijing Key Laboratory of Environment Friendly Management on Fruit Disease and Pests in North China,Institute of Plant Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Department of Plant Pathology,China Agricultural University,Beijing 100193,China)

机构地区：[1]北方果树病虫害绿色防控北京市重点实验室,北京市农林科学院植物保护研究所,北京100097 [2]中国农业大学植物保护学院,北京100193

出　　处：《植物病理学报》2023年第4期725-728,共4页Acta Phytopathologica Sinica

基　　金：财政部和农业农村部:国家现代农业产业技术体系(CARS-29)。

摘　　要：葡萄黑根病(Black foot disease)是重要的葡萄枝干病害之一,全球葡萄主要种植区均有发生[1],近两年我国有该病害发生的报道[1-2]。田间,黑根病主要危害8年树龄以下的幼树,症状主要表现为发芽异常,枝条节间缩短,叶片失绿黄化,根部出现褐色至黑色坏死等[3-5]。引起葡萄黑根病的病原菌复杂多样,已报道8个属34种病原菌[1]。Black foot disease is one of the most important grapevine trunk diseases in the world.In this study,three samples from diseased grapevine trunks were collected from three vineyards in Beijing municipality and Hebei province.The potential pathogen was isolated,purified,and identified.In addition,pathogenicity of the isolates was tested to fulfill Koch′s postulates,and the effect of temperature on their mycelial growth was investigated.According to morphological characteristics and a multi-gene phylogenetic analysis with sequences of ITS,EF 1-α,HIS3,and TUB2,five isolates were identified as Dactylonectria novozelandica,which was the causal agent of grapevine black foot disease.Moreover,the pathogen was able to grow between 10 and 30℃.The optimum temperature for mycelial growth was 25℃.This is the first report of grapevine black foot disease caused by D.novozelandica in China.

关 键 词：Grapevine black foot disease PATHOGENS IDENTIFICATION Dactylonectria novozelandica 

分 类 号：S436.631[农业科学—农业昆虫与害虫防治]