不同比例聚乳酸/丝素蛋白复合支架在兔尿道缺损修复中的疗效  

Comparison of the fficacy of composite scaffolds with different proportions of polylactic acid and silk fibroin in urethral repair in rabbits

作　　者：钟文文 李科[2] 刘碧好 蔡炳[3] 脱颖[4] 叶雷 马波[1] 瞿虎[1] 汪中扬[1] 王德娟 邱剑光 Zhong Wenwen;Li Ke;Liu Bihao;Cai Bing;Tuo Yin;Ye Lei;Ma Bo;Qu Hu;Wang Zhongyang;Wang Dejuan;Qiu Jianguang(Deparment of Urology,the Sixth Afiliaed Hospital of Sun Yat-sen University,Guangzhou 510655,China;Department of Urology,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China;Department of Reproductive Medicine,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510062,China;Department of Pathology,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510062,China)

机构地区：[1]中山大学附属第六医院泌尿外科,广州510655 [2]中山大学附属第三医院泌尿外科,广州510630 [3]中山大学附属第一医院生殖医学科,广州510062 [4]中山大学附属第一医院病理科,广州510062

出　　处：《中华腔镜泌尿外科杂志(电子版)》2023年第5期516-522,共7页Chinese Journal of Endourology(Electronic Edition)

基　　金：广东省自然科学基金面上项目(2019A1515010386)。

摘　　要：目的对比不同比例的3D打印聚乳酸及丝素蛋白复合支架在免尿道缺损修复中的疗效。方法构建聚乳酸(PLA)/丝素蛋白(SF)比例分别为25:75、50:50、75:25的复合支架材料,与诱导多能干细胞共培养。24只人工尿道下裂模型的雄性新西兰兔随机分为3组,分别采用不同比例的复合支架行尿道成形术。在尿道成形术后6周,收集尿道移植物并进行大体和组织学分析,统计尿道狭窄的发生率。结果体外实验中随着SF在复合材料中的比例增高,细胞黏附、增殖的数量增加。术后6周,PLA/SF=25:75比例组尿道狭窄发生率低于其他两组(P<0.05)。结论25:75比例的聚乳酸/丝素蛋白复合材料更加有助于尿道缺损的修复。Objective To compare the effect of 3D printing composite scaffolds with different proportions of polylactic acid(PLA)and silk fibroin(SF)in urethral repair in rabbits.Methods The composite scaffolds were built with different ratios of PLA and SF(25:75,50:50,75:25),and the Urethral epithelial cells that from stem cells were co-cultured on the composite scaffolds.24 male rabbits with artificial hypospadias were randomly divided into 3 groups,and treated by urethroplasty with different composite scaffolds.Urethral grafts were collected for histological analysis 6 weeks after urethroplasty,and the incidence of urethral stricture was calculated.ResultsAs the ratios of SF in the composite increased,the number of cells that adhered to the composite and propagated on the composite increased in vitro.Six weeks after urethroplasty,the incidence of urethral stricture in the group that proportion of PLA and SF 25:75 was lower than that in the other two groups(P<0.05).Conclusion The composite scaffolds,which proportion of PLA and SF was 25:75,were more helpful for urethral defect repair.

关 键 词：聚乳酸/丝素蛋白 3D打印 复合支架 尿道缺损 尿道修复 组织工程 再生医学 修复材料 

分 类 号：R699[医药卫生—泌尿科学]