组氨酸和五乙烯六胺功能化掺硼石墨烯量子点的合成及荧光检测中草药中姜黄素  

Synthesis of histidine and pentaethylenehexamine-functionalized and borondoped graphene quantum dots and its application in fluorescence detection of curcumin in Chinese herbal medicine

作　　者：许应生[1] 张花花 李瑞怡 徐鹏武 李在均 XU Yingsheng;ZHANG Huahua;LI Ruiyi;XU Pengwu;LI Zaijun(Department of Pharmacy,West Anhui Health Vocational College,Lu'an 237005,China;School of Life Science and Health Engineering,Jiangnan University,Wuxi 214122,China;School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,China)

机构地区：[1]皖西卫生职业学院药学系,六安237005 [2]江南大学生命科学与健康工程学院,无锡214122 [3]江南大学化学与材料工程学院,无锡214122

出　　处：《分析试验室》2023年第9期1150-1157,共8页Chinese Journal of Analysis Laboratory

基　　金：中国博士后科学基金(2021M702770);国家自然科学基金(52103032)项目资助。

摘　　要：将柠檬酸、组氨酸、五乙烯六胺和硼酸混合后热解,制备组氨酸和五乙烯六胺功能化掺硼石墨烯量子点(HPB-GQD)。所形成的HPB-GQD由片径4.17±0.12 nm的石墨烯片组成。石墨烯片边缘含有丰富的功能基团。HPB-GQD的荧光发射依赖于激发波长,375 nm的紫外光激发产生最强蓝色荧光发射。荧光量子产率达到87.4%,发光效率优于传统GQD以及单一组氨酸、五乙烯六胺或硼酸功能化GQD,证明组氨酸和五乙烯六胺以及硼的引入提高了GQD发光效率。基于姜黄素对HPB-GQD的荧光猝灭作用,建立了测定姜黄素的荧光分析方法。线性范围和检出限分别是0.05~20.0μmol/L和0.017μmol/L。方法已应用于中草药中姜黄素的荧光检测,结果与液相色谱-质谱法(LC-MS)结果基本一致,加标回收率在96.0%~104.0%之间。Citric acid,histidine,pentaethylenehexamine and boric acid were mixed and pyrolyzed to prepare histidine and pentaethylenehexamine-functionalized and boron-doped graphene quantum dots(HPB-GQD).The resulting HPB-GQD was composed of graphene sheets with size of 4.17±0.12 nm,and also with rich functional groups at the edges of graphene sheets.The fluorescence emission of HPB-GQD depended on the excitation wavelength.Ultraviolet excitation at 375 nm produced the strongest blue fluorescence emission.The fluorescence quantum yield was 87.4%,which was significantly better than that of traditional GQD,and single histidine,pentaethylenehexamine or boric acid-functionalized GQD,showing that introduction of histidine,pentaethylenehexamine and boron can significantly improve the luminescence efficiency.Based on the fluorescence quenching by the interaction between curcumin and HPB-GQD,a method for fluorescence determination of curcumin was established.The linear range and detection limit were 0.05-20.0μmol/L and 0.017μmol/L,respectively.The proposed method has been successfully applied to the fluorescence detection of curcumin in Chinese herbal medicine.The results were basically consistent with those of liquud chromatographymass spectrometry(LC-MS)and the recoveries were in the range of 96.0%-104.0%.

关 键 词：石墨烯量子点 荧光探针 姜黄素 

分 类 号：O657.31[理学—分析化学] X832[理学—化学]