铜离子掺杂双金属纳米球免疫传感器检测前列腺特异性抗原  

Detection of prostate specific antigen by electrochemical immunosensor with Cu^(2+)doped bimetallic nanospheres

作　　者：尹起 姜丽萍 郭丰佳 滕圆圆 韩洪文[1] 孙静波[1] 郭立娜[1] YIN Qi;JIANG Liping;GUO Fengjia;TENG Yuanyuan;HAN Hongwen;SUN Jingbo;GUO Lina(College of Engineering,Yantai Nanshan University,Yantai 265713,China;Shandong Nanshan Aluminum Co.,Ltd.,Yantai 264006,China;Dongchangfu District Maternal and Child Health Hospital,Liaocheng 252000,China)

机构地区：[1]烟台南山学院工学院,烟台265713 [2]山东南山铝业有限公司,烟台264006 [3]东昌府区妇幼保健院,聊城252000

出　　处：《分析试验室》2023年第9期1229-1235,共7页Chinese Journal of Analysis Laboratory

基　　金：山东省自然科学基金(ZR2019PB010);龙口市科技研发计划(2020KJJH061);南山集团科技计划项目(2022HX004);烟台南山学院青年基金(2021QKJ06)资助。

摘　　要：以比表面积大、结合位点多的金铂纳米球杂化二氧化锡石墨烯(GS-SnO2@Au-Pt)修饰玻碳电极,作为传感平台固定捕获抗体(Ab1),铜离子掺杂金银纳米球(Au-Ag@Cu^(2+))与检测抗体(Ab2)结合作为免疫探针,构建夹心型电化学免疫传感器,并用于检测前列腺特异性抗原(PSA)。基于Cu^(2+)和Cu^(+)之间的电子转移,以及金银双金属协同效应增强检测信号,通过方波伏安法(SWV)检测,在0.25 V处获得尖锐信号峰。结果表明,该免疫传感器具有较宽的线性范围(1 fg/mL~10 ng/mL)和较低的检出限(0.34 fg/mL),在PSA临床检测中有潜在应用前景。A sandwich-type electrochemical immunosensor for detecting prostate specific antigen(PSA)was reported.The Au-Pt nano-particles functionalized tin dioxide graphene(GS-SnO_(2)@Au-Pt)with larger specific surface and more binding sites was used to modify the electrode to provide binding sites for capturing antibodies(Ab_1)immobilization.The copper ions doped Au-Ag nanospheres(Au-Ag@Cu^(2+))were used to construct immune probes with labeled antibodies(Ab_(2)),and a sharp electrochemical signal peak at 0.25 V based on the electron transfer between Cu^(2+)/Cu^(+)and the synergistic effect of Au-Ag bimetallic was obtained by square wave voltammetry(SWV).The results showed that fabricated immunosensor exhibited a wide linear range(1 fg/mL to 10 ng/mL)with a low detection limit of 0.34 fg/mL.The immunosensor provided a promising way in clinic detection of PSA.

关 键 词：前列腺特异性抗原 铜离子掺杂金银纳米球 金铂纳米球杂化二氧化锡石墨烯 电化学免疫传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]