基于UPLC-Q-Exactive Orbitrap-MS技术分析胀果甘草地上部分的黄酮类化合物  

Analysis of flavonoid constituents in the aerial parts of Glycyrrhiza inflata Batalin.by UPLC-Q-Exactive Orbitrap-MS

作　　者：侯冰燕 张志 刘颖[1] 贾栖 云鹭 王文全[1,2,3] 侯俊玲[1,2] 彭一峰 HOU Bingyan;ZHANG Zhi;LIU Ying;JIA Xi;YUN Lu;WANG Wenquan;HOU Junling;PENG Yifeng(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs,Ministry of Education,Beijing 102488,China;Institute of Medicinal Plant Development,Chinese Academy of Medicinal Science and Peking Union Medical College,Beijing 100193,China;Xinjiang Quan an Pharmaceutical Limited Company,Korla 841011,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]中药材规范化生产教育部工程研究中心,北京102488 [3]中国医学科学院北京协和医学院药用植物研究所,北京100193 [4]新疆全安药业股份有限公司,库尔勒841011

出　　处：《西北药学杂志》2023年第6期1-14,共14页Northwest Pharmaceutical Journal

基　　金：国家重点研发计划资助项目(编号:2018YFC1706500)。

摘　　要：目的鉴定胀果甘草地上部分的黄酮类化合物,为后续药效及质量评价与控制等的研究奠定基础。方法用超高效液相色谱-四级杆-静电场轨道阱(UPLC-Q-Exactive Orbitrap-MS)联用技术,用Waters Acquity UPLC BEH-C 18色谱柱(100 mm×2.1 mm,1.7μm),以乙腈(A)-5 mL·L^(-1)甲酸水溶液(B)为流动相,梯度洗脱,流速为0.2 mL·min^(-1),在电喷雾正负离子模式下采集数据。依据高分辨质谱提供的准分子离子峰和碎片离子的精确分子质量信息及相关文献数据,确证黄酮类化合物及其特征碎片离子的分子组成。结果鉴定出胀果甘草地上部分的乙醇提取物共含有95个黄酮类化合物,其中水溶性部位有52个黄酮,主要以黄酮苷类为主;脂溶性部位有83个黄酮类化合物,主要为黄酮苷元类。水溶性和脂溶性部位含37个共有黄酮,主要为黄酮苷、二氢黄酮。结论胀果甘草地上部分含有多种黄酮类化合物,用UPLC-Q-Exactive Orbitrap-MS技术可以快速、准确、灵敏地鉴别胀果甘草地上部分的黄酮类化合物的结构。Objective To identify the flavonoid constituents in the aerial parts of Glycyrrhiza inflata Batalin.,so as to provide a research basis for the follow-up efficacy research and quality evaluation and control.Methods Waters Acquity UPLC BEH-C 18 chromatographic column(100 mm×2.1 mm,1.7μm)was used by using UPLC-Q-Exactive Orbitrap-MS technique with acetonitrile(A)-5 mL·L^(-1)formic acid solution(B)as the mobile phase for gradient elution.The flow rate was 0.2 mL·min^(-1),and the data were collected in the positive and negative ion modes of electric spray.The molecular composition of flavonoids and their characteristic fragment ions were confirmed based on the accurate molecular weight information of excimer ion peaks and fragment ions provided by high-resolution mass spectrometry and the support of relevant literature data.Results The ethanol extract of the aerial parts of Glycyrrhiza inflata Batalin.contained 95 flavonoids,including 52 flavonoids in the water-soluble parts,mainly flavonoid glycosides,83 flavonoids in the fat-soluble parts,mainly flavonoid aglycones.The water-soluble and fat-soluble parts contained 37 flavonoids,mainly flavonoid glycosides and dihydroflavones.Conclusion The aerial parts of Glycyrrhiza inflata Batalin.contains a variety of flavonoids.UPLC-Q-Exactive Orbitrap-MS technique can quickly,accurately and sensitively identify the chemical composition and structure of flavonoids in the aerial parts of Glycyrrhiza inflata Batalin.

关 键 词：胀果甘草 化学成分 黄酮类 超高效液相色谱-四级杆-静电场轨道阱 

分 类 号：R284.1[医药卫生—中药学]