姬松茸新品种“中菌姬松茸1号”的基因组Survey分析  

Genome Survey Analysis of New Variety ZJJSR001 of Agaricus blazei

作　　者：李雪松 李建英[1] 华蓉[1,2] 刘绍雄 岳万松[1] 张晓华 高章会 岳婷松 孙达锋 LI Xue-song;LI Jian-ying;HUA Rong;LIU Shao-xiong;YUE Wan-song;ZHANG Xiao-hua;GAO Zhang-hui;YUE Ting-song;SUN Da-feng(Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooperatives,Kunming 650221,China;Yunnan Academy of Edible Fungi Industry Development,Kunming 650221,China)

机构地区：[1]中华全国供销合作总社昆明食用菌研究所,云南昆明650221 [2]云南省食用菌产业发展研究院,云南昆明650221

出　　处：《中国食用菌》2023年第5期47-51,57,共6页Edible Fungi of China

基　　金：国家重点研发计划课题(2022YFD1200602);云南省科技人才与平台计划(202305AD160051)。

摘　　要：为了深入开展姬松茸子实体发育、重金属富集等遗传机制的研究,为姬松茸基因组的遗传精细图谱、分子育种等研究奠定基础,采用第二代高通量测序技术对姬松茸新品种“中菌姬松茸1号”开展全基因组Survey分析。结果表明,姬松茸新品种“中菌姬松茸1号”的基因组大小约为46.59 Mb,测序深度为69×,杂合率为1.37%,(G+C)含量为46.87%,重复序列比例为24.46%;基因组初步组装后,得到20342条重叠序列(Scaffold),Scaffold N50为4.29 Kb。In order to study the genetic mechanism of Agaricus Blazei fruit body development and heavy metal accumulate me-chanicm,it will lay a fundamental research of A.Blazei genome survey to genetic map and molecular breeding,the genome of A.blazei was measured by next-generation sequencing.The results showed that the genome size of A.blazei was 46.59 Mb and the sequencing depth was 69×,the heterozygosity rate was 1.37%,(G+C)content was 46.87%.The proportion of repeated sequences was 24.46%.20342 scaffolds were obtained which was assembled and the scaffold N50 was 4.29 Kb.

关 键 词：姬松茸 全基因组survey 杂合率 基因组大小 GC含量 

分 类 号：S646.1[农业科学—蔬菜学]