基于点扫描的高时空分辨荧光显微成像技术进展  

Advances in high spatiotemporal resolution fluorescence microscopic imaging technique based on point scanning

作　　者：潘彬雄 弓晟 张鹏 刘子叶 皮彭健 陈旺 黄文强 王保举 詹求强 Pan Bin-Xiong;Gong Cheng;Zhang Peng;Liu Zi-Ye;Pi Peng-Jian;Chen Wang;Huang Wen-Qiang;Wang Bao-Ju;Zhan Qiu-Qiang(South China Academy of Advanced Optoelectronics,South China Normal University,Guangzhou 510006,China;School of Physics,South China Normal University,Guangzhou 510006,China)

机构地区：[1]华南师范大学,华南先进光电子研究院,广州510006 [2]华南师范大学物理学院,广州510006

出　　处：《物理学报》2023年第20期298-313,共16页Acta Physica Sinica

基　　金：国家自然科学基金(批准号:62335008,62122028,11974123,62105106);广东省基础与应用基础研究基金(批准号:2023B1515040018,2022A1515011395);广州市基础与应用基础研究基金(批准号:202201010376);中国博士后科学基金(批准号:2021M691089,2023T160237)资助的课题。

摘　　要：激光点扫描荧光显微镜凭借高分辨率、高灵敏度、高特异性、可光学层切三维成像和动态成像等优势,已成为生命科学研究中广泛应用的重要工具.随着研究者对生命科学研究的逐渐深入,由于受光学衍射极限影响和逐点扫描探测的限制,传统点扫描共聚焦显微镜的时空分辨率已无法完全满足相关研究需求,在实际应用中面临诸多挑战.近二十年来,超分辨荧光显微成像技术取得了重要进展,研究人员发展了多种高空间分辨率、高时间分辨率的点扫描荧光显微成像技术,对于生物成像等具有重要意义.然而,有关该领域最新进展的综述论文较少,系统地讨论、总结基于点扫描的高时空分辨荧光显微成像技术的研究进展,对于其未来研究发展极为重要.本文主要从时间分辨率与空间分辨率两个维度分别介绍了各类点扫描荧光显微成像技术的基本原理及相关进展,并介绍了高时空分辨显微成像技术及应用,最后讨论展望了高时空分辨点扫描荧光显微镜的未来发展趋势和挑战.Laser point-scanning fluorescence microscopy serves as an indispensable tool in the life science research,owing to its merits of excellent resolution,high sensitivity,remarkable specificity,three-dimensional opticalsectioning capability,and dynamic imaging.However,conventional laser point-scanning fluorescence microscopy confronts a series of challenges in the rapidly evolving field of life sciences,because of the limitations imposed by optical diffraction and point scanning detection.Over the past two decades,substantial advancements have been made in super-resolution fluorescence microscopic imaging techniques.Researchers have developed various high spatial and temporal resolution point-scanning microtechniques,which hold great significance for biological optical imaging and other relevant applications.Regrettably,there are still few review articles covering the recent progress of this field.It is essential to provide a comprehensive review of laser point-scanning fluorescence microscopic techniques for their future developments and trends.In this article,the basic principles and recent advances in different point-scanning fluorescence microscopy imaging techniques are introduced from the perspectives of temporal resolution and spatial resolution,and the progress and applications of high spatiotemporal resolution microscopic imaging techniques based on point-scanning mode are summarized.Finally,the development trends and challenges of high spatio-temporal resolution point scanning fluorescence microscopic imaging technique are discussed.

关 键 词：点扫描共聚焦 超分辨显微成像 荧光显微镜 时空分辨率 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术] TH742.65[自动化与计算机技术—计算机科学与技术]