一株临沧覆土栽培盖囊菇高温品种可控环境因子优化研究  

Optimization of Controllable Environmental Factors of a High-temperature Variety of Pleurotus cystidiosus Cultivated by Soil Covering in Lincang,Yunnan,China

作　　者：洪鹏 和敏 周会明 张焱珍 卯明娟 王瑞 马凯云 邓云 HONG Peng;HE Min;ZHOU Huiming;ZHANG Yanzhen;MAO Mingjuan;WANG Rui;MA Kaiyun;DENG Yun(School of Biotechnology and Engineering,West Yunnan University,Lincang,Yunnan 677000,China)

机构地区：[1]滇西科技师范学院生物技术与工程学院,云南临沧677000

出　　处：《热带作物学报》2023年第10期2060-2072,共13页Chinese Journal of Tropical Crops

基　　金：云南省教育厅科学研究基金项目(No.2022J1018);国家食用菌产业技术体系资助项目(No.CARS-20)。

摘　　要：在马铃薯蔗糖琼脂培养基的基础上,采用单因素与L_(16)(4^(5))正交优化试验的方法,研究5种环境条件对盖囊菇菌丝体生长与孢梗束形成的影响。结果表明:盖囊菇菌丝体在温度10~30℃、琼脂浓度0.5%~3.0%、pH 4.00~10.00、培养皿直径60~100 mm、光照时间0~24 h的范围内均能生长,其中在温度25℃、琼脂浓度3.0%、pH 5.80、培养皿直径100 mm、黑暗条件下菌丝体生长最快。盖囊菇孢梗束在15~30℃温度范围内均能形成,其余环境条件与其菌丝体相同,在温度27℃、琼脂浓度2.0%、pH 8.00、培养皿直径100 mm、光照时长24 h下孢梗束形成数量最多。正交优化试验表明,盖囊菇菌丝体生长最适环境条件组合为:温度25℃、琼脂浓度3.0%、pH 5.80、培养皿直径100 mm、光照时长0 h;其孢梗束形成最适环境条件为:温度25℃、琼脂浓度2.0%、pH 8.00、培养皿直径100 mm、光照时长14 h。总之,环境条件对盖囊菇菌丝体生长与孢梗束形成的影响较大且存在交互作用。In this paper,on the basis of potato sucrose agar medium,the single factor and L_(16)(4^(5))orthogonal optimization experiments were used to study the effects of 5 environmental factors on the mycelial growth and coremium formation of P.cystidiosus.The results showed that the mycelium of P.cystidiosus could grow in the range of temperature 10-30℃,agar concentration 0.5%-3.0%,pH 4.00-10.00,petri dish diameter 60-100 mm,and light time 0-24 h.The my-celium grew the fastest under the conditions of temperature 25℃,agar concentration 3.0%,pH 5.80,petri dish diameter 100 mm and dark conditions.The coremium of P.cystidiosus can be formed in the range of temperature 15-30℃,and the other environmental conditions were the same as that of its mycelium.The number of coremium formation was the largest under pH 8.00,petri dish diameter 100 mm and light duration of 24 h.The orthogonal optimization test showed that the optimum environmental conditions for mycelium growth of P.cystidiosus were temperature 25℃,agar concen-tration 3.0%,pH 5.80,petri dish diameter 100 mm,and dark conditions.The optimum environmental conditions for its coremium formation were temperature 25℃,agar concentration 2.0%,pH 8.00,petri dish diameter 100 mm,and light duration 14 h.In a word,the environmental conditions had a great influence on the mycelium growth and coremium formation of P.cystidiosus,and there was an interaction.

关 键 词：盖囊菇 正交试验 可控环境因子 菌丝体生长 孢梗束形成 

分 类 号：S646.2[农业科学—蔬菜学]