喹诺酮配合物材料的制备及其抗菌性能研究  

Preparation of quinolone complexes and their antibacterial properties study

作　　者：夏姣云[1] 李潇潇 徐彤 杨灿 XIA Jiaoyun;LI Xiaoxiao;XU Tong;YANG Can(School of Chemistry and Chemical Engineering,Changsha University of Science and Technology,Changsha,410114,China)

机构地区：[1]长沙理工大学化学化工学院,长沙410114

出　　处：《功能材料》2023年第10期10216-10223,共8页Journal of Functional Materials

基　　金：湖南省自然科学基金项目(2018JJ2422)。

摘　　要：以环丙沙星、5-甲基-2-(2′-吡啶基)苯并咪唑为原料合成喹诺酮类配合物[Cu(SF)(HPBM)](NO_(3))_(2)·(H_(2)O)1.5,(SF=环丙沙星,HPBM=5-甲基-2-(2′-吡啶基)苯并咪唑),通过红外、紫外、元素、质谱分析对配合物进行表征。由MTT检测法、平板涂布法、滤纸片法测试了配合物及配体对大肠杆菌和金黄色葡萄球菌的抗菌性能,验证了配合物SF-Cu-HPBM的抗菌性能是大于配体的,并且通过紫外、DNA-EB体系与荧光光谱分析,研究了配合物与小牛胸腺DNA的相互作用。结果表明,配合物都是以经典插入模式与DNA结合。探究配合物与牛血清白蛋白(BSA)作用机制,发现配合物与BSA的结合常数为2.36×10^(5)M^(-1),明显高出第一配体环丙沙星与BSA的结合常数3.85×10^(3)M^(-1),实验数据表明配合物与BSA更易结合。Synthesis of quinolone complexes[Cu(SF)(HPBM)](NO_(3))_(2)·(H_(2)O)1.5,(SF=ciprofloxacin,HPBM=5-methyl-2-(2'-pyridyl)benzimidazole),characterization of complexes by infrared,ultraviolet,elemental,mass spectrometry.The antibacterial properties of complexes and ligands against Escherichia coli and Staphylococcus aureus were tested by MTT detection,plate coating and filter paper sheet method,and the antibacterial performance of the complex SF-Cu-HPBM was greater than that of the ligand,and the interaction between the complex and calf thymus DNA was studied by ultraviolet,DNA-EB system and fluorescence spectroscopy,and the results showed that the complexes were bound to DNA in classical insertion mode.Exploring the mechanism of action between complexes and bovine serum albumin(BSA),it was found that the binding constant of the complex to BSA was 2.36×10^(5)M^(-1),which was significantly higher than that of the first ligand ciprofloxacin and BSA by 3.85×10^(3)M^(-1),and the experimental data showed that the complex and BSA were easier to bind.

关 键 词：喹诺酮类配合物 抗菌性能 DNA作用机制 溴化乙锭竞争实验 牛血清白蛋白作用机制 

分 类 号：O614.121[理学—无机化学]