宜昌荚蒾叶片诱导愈伤组织及植株再生体系的建立  

Leaf Induced Callus and the Establishment of Plant Regeneration System of Viburnum erosum Thunb.

作　　者：孙晓慧 吕洪岩 孙超 于晓明 宋黎 徐涛 董爱新 陈甘牛 SUN Xiaohui;LV Hongyan;SUN Chao;YU Xiaoming;SONG Li;XU Tao;DONG Aixin;CHEN Ganniu(Yantai Forest Resources Monitoring and Protection Service Center,Yantai 264001;Dong'e County Forestry Development Center,Liaocheng 252299;Shandong Academy of Forestry,250014)

机构地区：[1]烟台市森林资源监测保护服务中心,山东烟台264001 [2]东阿县林业发展中心,山东聊城252299 [3]山东省林业科学研究院,山东济南250014

出　　处：《山东林业科技》2023年第5期54-57,62,共5页Journal of Shandong Forestry Science and Technology

摘　　要：以宜昌荚蒾叶片作为外植体,研究了培养基种类、不同配比的植物生长调节剂对宜昌荚蒾叶片愈伤组织诱导和小植株再生的影响。结果表明:宜昌荚蒾叶片灭菌最适方法为75%酒精处理30s,再用0.1%HgCl_(2)溶液处理5 min;叶片诱导愈伤组织的最适培养基为1/2MS+6-BA0.05 mg·L^(-1)+NAA1.0 mg·L^(-1),诱导率100%;再分化的最佳培养基为N6+6-BA0.5 mg·L^(-1)+TDZ0.7mg·L^(-1)+IBA0.5 mg·L^(-1)+IAA0.2 mg·L^(-1)再分化率为100%;最佳生根培养基为1/2MS+6-BA0.05 mg·L^(-1)+NAA0.50 mg·L^(-1)+活性炭1 g·L^(-1),生根率为100%。Using the leaves of Viburnum erosum Thunb.as explants,the effects of medium types and different ratios of plant growth regulators on callus induction and small plant regeneration of V.erosum Thunb.leaves were studied.The research results indicate that the optimal method for sterilization of V.erosum.The leaves of V.erosum is to treat with 75%alcohol for 30 seconds,and then treat with a 0.1%HgCl_(2) solution for 5 minutes;The optimal medium for callus induction is 1/2MS+6-BA0.05 mg·L^(-1)+NAA1.0 mg·L^(-1),with an induction rate of 100%;The optimal medium for redfferentiation is N6+6-BA0.5 mg·L^(-1)+TDZ0.7 mg·L^(-1)+IBA0.5 mg·L^(-1)IAA0.2 mg·L^(-1+),with a 100%redifferentiation rate;The optimal rooting culture medium is 1/2MS+6-BA0.05 mg·L^(-1)+NAA0.50 mg·L^(-1)+activated carbon 5 g·L^(-1),with a rooting rate of 100%.

关 键 词：宜昌荚蒾 叶片 愈伤组织 再分化 

分 类 号：S685.99[农业科学—观赏园艺]