儿童流感样症状病原学特点与肠道菌群分布特征的研究  

Study on etiological characteristics and intestinal flora distribution in children with influenza-like symptoms

作　　者：葛飞霞 章铮 郭平安 GE Fei-xia;ZHANG Zheng;GUO Ping-an(The First People's Hospital of Fuyang District,Zhejiang 311400,China)

机构地区：[1]杭州市富阳区第一人民医院,浙江311400

出　　处：《中国卫生检验杂志》2023年第18期2260-2263,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的探讨流感样症状儿童患者病毒病原学检测及流行病学分布与肠道菌群分布特征的相关性。方法收集2020年1月—2022年12月因流感样症状来本院就诊800例患儿鼻咽拭子标本,采用逆转录聚合酶链反应法(RT-PCR)对6种呼吸道病毒核酸进行检测;随机选择部分不同病毒病原菌患儿检测肠道菌群。结果800例流感样疾病患儿标本共检出呼吸道病毒339例(42.4%),其中单纯感染301例(37.6%),多重感染38例(4.8%)。呼吸道合胞病毒(RSV)检出率居首位,为17.4%(139/800),阳性率高于其他病原菌(P<0.05)。各年龄组间病毒检出率差异有统计学意义(P<0.05),以3岁~<6岁组病毒检出率最高(52.4%,150/286)。不同病原学感染流感样患儿肠道双歧杆菌、嗜酸杆菌、肠球菌、大肠杆菌检测结果差异无统计学意义(P>0.05)。结论流感性症状患儿常见于单纯的呼吸道病毒感染且以RSV感染为主,RSV、FluB和ADV感染呈现一定的年龄分布;不同病原菌感染患儿肠道菌群分布特征无明显差异。Objective This paper aims to explore the correlation between the detection and epidemiological distribution of viral pathogens in children with influenza-like symptoms and the distribution of intestinal flora.Methods Nasopharyngeal swab samples from 800 children with influenza-like symptoms who came to our hospital from January 2020 to December 2022 were collected.Six kinds of respiratory virus nucleic acids were detected by reverse transcription polymerase chain reaction(RT-PCR).Some children with different viral pathogens were randomly selected to detect intestinal flora.Results A total of 339 cases(42.4%)of respiratory virus were detected in 800 children with influenza-like diseases,including 301 cases(37.6%)of simple infection and 38 cases(4.8%)of mixed infection.The detection rate of respiratory syncytial virus(RSV)was 17.4%(139/800),which was significantly higher than other pathogenic bacteria(P<0.05).There was statistical signifi⁃cance on the difference in the virus detection rates among the age groups(P<0.05),with the highest detection rate in the 3-<6 age group(52.4%,150/286).There was no statistical significance on the difference in the detection results of intesti⁃nal Bifidobacteria,Acidophilic bacilli,Enterococci and Escherichia coli in children with influenza-like infection from different pathogens(P>0.05).Conclusion Influenza symptoms are common in children with simple respiratory tract virus infection and mainly RSV infection.RSV,FluB and ADV infections present in certain ages.There is no significant difference in the dis⁃tribution of intestinal flora in children infected by different pathogens.

关 键 词：儿童 流感样疾病 呼吸道病原菌 肠道菌群 

分 类 号：R511.7[医药卫生—内科学]