UPLC-MS/MS法同时测定补中益气丸(水丸)中6种化合物含量  

Simultaneous Determination of Six Components in Buzhong Yiqi Pills(Water Pills)by UPLC-MS/MS

作　　者：田小权 张加稳 王葳 TIAN Xiaoquan;ZHANG Jiawen;WANG Wei(Kunming Center for ADR and Drug Abuse Monitoring,Kunming 650000,China;Kunming Food and Drug Inspection Institute,Kunming 650000,China;Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]昆明市药品不良反应与药物滥用监测中心,云南昆明650000 [2]昆明市食品药品检验所,云南昆明650000 [3]云南中医药大学,云南昆明650500

出　　处：《云南中医药大学学报》2023年第5期49-53,共5页Journal of Yunnan University of Chinese Medicine

基　　金：云南中医药大学研究生优质课程建设项目-天然药物质量评价技术。

摘　　要：目的建立UPLC-MS/MS法同时测定补中益气丸(水丸)中黄芪甲苷、毛蕊异黄酮苷、橙皮苷、阿魏酸、甘草酸和甘草苷的含量。方法采用Agilent Zorbax Eclipse XDB-C18色谱柱(100 mm×2.1 mm,1.8μm),流动相为5 mmol/L乙酸铵溶液-甲醇(含0.1%甲酸),梯度洗脱;质谱离子化方式为电喷雾源(ESI),采用多级反应离子监测模式(MRM)定量分析。结果各化合物在相应浓度范围内线性关系均良好,r^(2)值为0.994~0.999,加标回收率范围为86.7%~103.5%,相应的RSD为3.8%~7.6%。结论本方法定性和定量准确可靠,重复性好、基质干扰小、灵敏度高,满足补中益气丸(水丸)中6种化合物含量的测定要求。Objective To establish a UPLC-MS/MS method for the simultaneous determination of astragaloside IV,verbascoside,hesperidin,ferulic acid,glycyrrhizic acid,and liquiritin in Buzhong Yiqi Pills(water pills).Methods Agilent Zorbax Eclipse XDB-C18 column(100 mm×2.1 mm,1.8μm)was used,and the mobile phase was 5 mmol/L ammonium acetate solution-acetonitrile(containing 0.1%formic acid),gradient elution;the ionization mode of mass spectrometry was electrospray source(ESI),and the quantitative analysis was carried out by using multiple reactive ion monitoring mode(MRM).Results Each compound had a good linear relationship in the corresponding concentration range,the r^(2)was between 0.994~0.999,the recovery rate of standard addition ranged from 86.7%to 103.5%,and the corresponding RSD was 3.8%to 7.6%.Conclusion This method is accurate and reliable qualitatively and quantitatively,with good repeatability,small matrix interference and high sensitivity,which meets the requirements of measuring the content of 6 components in Buzhong Yiqi Pills(water pills).

关 键 词：补中益气丸(水丸) 超高效液相色谱-串联质谱法 毛蕊异黄酮苷 黄芪甲苷 阿魏酸 橙皮苷 甘草苷 甘草酸 

分 类 号：R286.0[医药卫生—中药学]