流苏子根中化学成分的分离鉴定和抗菌活性研究  

Studies on the Isolation and Identification of Chemical Components from Coptosapelta diffusa Roots and Their Antimicrobial Activities

作　　者：白路遥 杨雪飞[2] 鲁春华[1] 沈月毛[1] BAI Luyao;YANG Xuefei;LU Chunhua;SHEN Yuemao(School of Pharmaceutical Sciences,Key Laboratory of Chemical Biology(Ministry of Education),Shandong University,Jinan 250012,China;Key Laboratory of Economic Plants and Biotechnology,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China)

机构地区：[1]山东大学药学院天然产物生物学教育部重点实验室,山东济南250012 [2]中国科学院昆明植物研究所植物资源与生物技术研究室,云南昆明650201

出　　处：《云南中医药大学学报》2023年第5期59-65,共7页Journal of Yunnan University of Chinese Medicine

基　　金：国家自然科学基金项目(22377067,22177062);教育部创新滚动支持项目(IRT_17R68);山东大学微生物技术国家重点实验室创新研究群体基金(WZCX2021-03)。

摘　　要：目的系统开展流苏子[Coptosapelta diffusa(Champ.ex Benth.)Steenis]根中化学成分的分离、鉴定和抗菌活性测试。方法利用硅胶、Sephadex LH-20等柱色谱方法进行分离、纯化,根据波谱数据鉴定化合物的结构,通过纸片扩散法进行抗菌活性测试。结果从流苏子根中分离得到12个化合物,分别鉴定为拉帕醇(lapachol,1)、hydroxyhydrolapachol(2)、dehydro-α-lapachone(3)、α-lapachone(4)、rhinacantin A(5)、β-lapachone(6)、2-carbomethoxyanthraquinone(7)、7-hydroxy-2-methylanthraquinone(8)、isoscopoletin(9)、alyxialactone(10)、(3S)-3-(hydroxymethyl)-3H-isobenzofuran-1-one(11)和(7’S,8S,8’R)-4,4’-dihydroxy-3,3’,5,5’-tetramethoxy-7,9-epoxylignan-9’-ol-7-one(12)。化合物1~4和6对测试病原菌菌株表现出较好的抗菌作用。结论化合物1~6和10~12均为首次从该种植物中分离得到;流苏子根中拉帕醇的含量为3.56 g/kg(干重),可作为拉帕醇的原料来源;该研究为流苏子根抗菌消炎的民间药用价值提供了科学依据。Objective To study the chemical constituents of Coptosapelta diffusa roots and measure their antimicrobial activities.Methods The chemical constituents were isolated and purified using silica gel,Sephadex LH-20 column chromatographies.Their structures were identified based on spectral analysis,and the antimicrobial activity was carried out by the paper diffusion method.Results 12 compounds were identified as lapachol(1),hydroxyhydrolapachol(2),dehydro-α-lapachone(3),α-lapachone(4),rhinacantin A(5),β-lapachone(6),2-carbomethoxyanthraquinone(7),7-hydroxy-2-methylanthraquinone(8),isoscopoletin(9),alyxialactone(10),(3S)-3-(hydroxymethyl)-3H-isobenzofuran-1-one(11),and(7’S,8S,8’R)-4,4’-dihydroxy-3,3’,5,5’-tetramethoxy-7’,9-epoxylignan-9’-ol-7-one(12).Compounds 1-4 and 6 showed good antimicrobial activity against the tested strains.Conclusion Compounds 4-6 and 10-12 were isolated from this plant for the first time.Lapachol was identified as the main constituent of C.diffusa roots with the content of 3.56 g/kg(dry weight),indicating that C.diffusa roots could serve as a source of lapachol.This study provided a scientific basis for the folk antimicrobial and anti-inflammatory medicinal function of C.diffusa roots.

关 键 词：流苏子根 化学成分 拉帕醇 抗菌活性 纸片扩散法 

分 类 号：R284.2[医药卫生—中药学]