HPLC同时测定三黄胶囊中11种成分的含量  

Simultaneous Determination of Eleven Components in Sanhuang Capsule by HPLC

作　　者：汪健 金顺琪 黄菲[1] 张露蓉[1] WANG jian;JIN Shunqi;HUANG fei;ZHANG Lurong(Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine,Suzhou 215007,China;Suzhou Hospital of Integrated Traditional Chinese and Western Medicine,Suzhou 215101,China)

机构地区：[1]南京中医药大学附属苏州市中医医院,江苏苏州215007 [2]苏州市中西医结合医院,江苏苏州215101

出　　处：《云南中医药大学学报》2023年第5期74-79,共6页Journal of Yunnan University of Chinese Medicine

基　　金：2020年度苏州市民生科技示范项目(SS202006);2020年度姑苏卫生人才培养项目(GSWS2020079)。

摘　　要：目的采用高效液相色谱法同时测定医院制剂三黄胶囊中11种成分的含量,作为其质量控制的方法。方法选择Agilent Extend-C18柱(250 mm×4.6 mm,5μm),将pH 2.5的甲醇-磷酸水溶液作为流动相,进行1mL·min^(-1)的梯度洗脱,检测波长和柱温为280 nm、30℃,吸取10μL进样。结果各成分的平均加样回收率为99.15%~116.70%。盐酸小檗碱、黄芩苷、槲皮素、汉黄芩苷、黄芩素、汉黄芩素、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的平均含量为(10.14±0.93)、(12.88±1.12)、(1.71±0.17)、(2.78±0.22)、(0.21±0.01)、(0.20±0.06)、(0.21±0.04)、(0.53±0.04)、(0.35±0.09)、(0.64±0.05)、(0.23±0.04)mg/粒。结论该方法简便、稳定、重复性良好,可作为三黄胶囊的质控标准。Objective To determine 11 components in Sanhuang Capsule by HPLC as a quality control method.Methods The chromatographic column was selected Agilent Extend-C18 column(250 mm×4.6 mm,5μm),methanolphosphoric acid aqueous solution with pH 2.5 was used as the mobile phase for gradient elution at a rate of 1 mL·min^(-1),the detection wavelength was 280 nm,the column temperature was 30℃and the injection volume was 10μL.Results The average recoveries were 99.15%-116.70%.The average content of berberine hydrochloride,baicalin,quercetin,wogonoside,baicalein,wogonin,aloe-emodin,rhein,emodin,chrysophanol,and physcion were(10.14±0.93)、(12.88±1.12)、(1.71±0.17)、(2.78±0.22)、(0.21±0.01)、(0.20±0.06)、(0.21±0.04)、(0.53±0.04)、(0.35±0.09)、(0.64±0.05)、(0.23±0.04)mg/grain.Conclusion The method is simple,stable and reproducible,and can be used as the quality control standard of Sanhuang Capsule.

关 键 词：三黄胶囊 高效液相色谱法 含量测定 

分 类 号：R286.0[医药卫生—中药学]